Skip to main content

Advertisement

SpringerLink
Log in

Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 × 105 cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Covas DT, Siufi JL, Silva AR, Orellana MD (2003) Isolation and culture of umbilical vein mesenchymal stem cells. Braz J Med Biol Res 36:1179–1183

    Article  PubMed  CAS  Google Scholar 

  • Doan N, Reher P, Meghji S, Harris M (1999) In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes. J Oral Maxillofac Surg 57: 409–419; discussion 420

    Google Scholar 

  • Erices A, Conget P, Minguell JJ (2000) Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 109:235–242

    Article  PubMed  CAS  Google Scholar 

  • Karahuseyinoglu S, Cinar O, Kilic E, Kara F, Akay GG, Demiralp DO, Tukun A, Uckan D, Can A (2007) Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem Cells 25:319–331

    Article  PubMed  CAS  Google Scholar 

  • Lu LL, Liu YJ, Yang SG, Zhao QJ, Wang X, Gong W, Han ZB, Xu ZS, Lu YX, Liu D (2006) Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. Haematologica 91:1017–1026

    PubMed  CAS  Google Scholar 

  • Mareschi K, Ferrero I, Rustichelli D, Aschero S, Gammaitoni L, Aglietta M, Madon E, Fagioli F (2006) Expansion of mesenchymal stem cells isolated from pediatric and adult donor bone marrow. J Cell Biochem 97:744–754

    Article  PubMed  CAS  Google Scholar 

  • Min BH, Woo JI, Cho HS, Choi BH, Park SJ, Choi MJ, Park SR (2006) Effects of low-intensity ultrasound (LIUS) stimulation on human cartilage explants. Scand J Rheumatol 35:305–311

    Article  PubMed  Google Scholar 

  • Mitchell KE, Weiss ML, Mitchell BM, Martin P, Davis D, Morales L, Helwig B, Beerenstrauch M, Abou-Easa K, Hildreth T (2003) Matrix cells from Wharton’s jelly form neurons and glia. Stem Cells 21:50–60

    PubMed  CAS  Google Scholar 

  • Nanaev AK, Kohnen G, Milovanov AP, Domogatsky SP, Kaufmann P (1997) Stromal differentiation and architecture of the human umbilical cord. Placenta 18:53–64

    Article  PubMed  CAS  Google Scholar 

  • Panepucci RA, Siufi JL, Silva WA Jr, Proto-Siquiera R, Neder L, Orellana M, Rocha V, Covas DT, Zago MA (2004) Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells. Stem Cells 22:1263–1278

    Article  PubMed  CAS  Google Scholar 

  • Pilla AA, Mont MA, Nasser PR, Khan SA, Figueiredo M, Kaufman JJ, Siffert RS (1990) Non-invasive low-intensity pulsed ultrasound accelerates bone healing in the rabbit. J Orthop trauma 4:246–253

    Article  PubMed  CAS  Google Scholar 

  • Sarugaser R, Lickorish D, Baksh D, Hosseini MM, Davies JE (2005) Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors. Stem Cells 23:220–229

    Article  PubMed  Google Scholar 

  • Seshareddy K, Troyer D, Weiss ML (2008) Method to isolate mesenchymal-like cells from Wharton’s Jelly of umbilical cord. Methods Cell Biol 86:101–119

    Article  PubMed  CAS  Google Scholar 

  • Sobolewski K, Malkowski A, Bankowski E, Jaworski S (2005) Wharton’s jelly as a reservoir of peptide growth factors. Placenta 26:747–752

    Article  PubMed  CAS  Google Scholar 

  • Weiss ML, Medicetty S, Bledsoe AR, Rachakatla RS, Choi M, Merchav S, Luo Y, Rao MS, Velagaleti G, Troyer D (2006) Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson’s disease. Stem Cells 24:781–792

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by a grant from Seoul Research and Business Development Program (10548).

Author information

Authors and Affiliations

  1. Department of Laboratory Medicine, Seoul National University Boramae Hospital, 425 Shindaebang-dong, Dongjak-gu, Seoul, 156-707, South Korea

    Jong Hyun Yoon, Eun Youn Roh & Sue Shin

  2. Seoul Metropolitan Public Cord Blood Bank (Allcord), Seoul, 156-707, South Korea

    Nam Hee Jung

  3. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, 110-744, South Korea

    Eun Young Song, Dong Soon Lee & Kyou Sup Han

  4. Department of Family Medicine, Seoul National University Boramae Hospital, Seoul, 156-707, South Korea

    Joung Sung Kim

  5. Department of Obstetrics and Gynecology, Seoul National University Boramae Hospital, Seoul, 156-707, South Korea

    Byoung Jae Kim & Hye Won Jeon

  6. Department of Orthopedic Surgery, Seoul National University Boramae Hospital, Seoul, 156-707, South Korea

    Kang Sup Yoon

Authors
  1. Jong Hyun Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eun Youn Roh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sue Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nam Hee Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eun Young Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dong Soon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kyou Sup Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Joung Sung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Byoung Jae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hye Won Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Kang Sup Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sue Shin.

Electronic supplementary material

Below is the link to the electronic supplementary material.

MOESM1 (DOC 28 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yoon, J.H., Roh, E.Y., Shin, S. et al. Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells. Biotechnol Lett 31, 329–335 (2009). https://doi.org/10.1007/s10529-008-9872-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-008-9872-5

Keywords

Search

Navigation