Abstract
(R)-Ethyl-3-hydroxyglutarate, (R)-3, is an intermediate in the synthesis of the statin side chain. Here, a new two-step, indirect biotransformation pathway involving the formation of ethyl (R)-4-carbamoyl-3-hydroxybutanoate, (R)-2, as an intermediate for (R)-3 production was developed using Rhodococcus boritolerans with ethyl (R)-4-cyano-3-hydroxybutyate, (R)-1, as substrate. Maximum conversion was with 10 g (R)-1/l, 7 g cells/l (dry wt), pH 7.5 and 25°C. A yield of 98 ± 0.5% (w/w) was attained within 8 h.
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Acknowledgments
This work was supported by the Natural Science Foundation of China (No. 309771427, 310717150), the National Key Project for Basic Research (No. 2010CB126102). Dr. Jidong Wang (Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, China) is thanked for NMR structure determinations of (R)-2 and (R)-3.
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Yang, MJ., Wang, XJ., Yang, ZY. et al. Bioconversion of ethyl (R)-4-cyano-3-hydroxybutyate into (R)-ethyl-3-hydroxyglutarate via an indirect pathway by Rhodococcus boritolerans . Biotechnol Lett 34, 901–905 (2012). https://doi.org/10.1007/s10529-012-0846-2
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DOI: https://doi.org/10.1007/s10529-012-0846-2