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A strong promoter, PMagpd, provides a tool for high gene expression in entomopathogenic fungus, Metarhizium acridum

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Abstract

A glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter (PMagpd) was obtained from Metarhizium acridum and its active region analyzed by 5′-deletion strategy using β-glucuronidase (GUS) as a reporter. Sequence analysis revealed that typical regulatory elements of PMagpd were included in the 1.7 kb region upstream of the start codon of the Magpd gene. Deletion of the region from −1,691 bp to −1,463 bp, where the gpd box is harbored, did not significantly affect the PMagpd activity. Deletions of the regions upstream of −946 bp and upstream of −684 bp caused a major decrease of GUS activity. Compared with PgpdA (2.2 kb) in Aspergillus nidulans, PMagpd (1.4 kb) had a shorter sequence and significantly higher activity in M. acridum. This study provides an applicable promoter for over-expression of target genes in M. acridum.

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Acknowledgments

This research was supported by grants from the Natural Science Foundation of China (No. 30800736) and Natural Science Foundation Project of CQ (CSTC2010BB5077).

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Authors and Affiliations

  1. Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides and Key Lab of Functional Gene and Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing, 400030, People’s Republic of China

    Yueqing Cao, Run Jiao & Yuxian Xia

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  1. Yueqing Cao
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  2. Run Jiao
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  3. Yuxian Xia
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Correspondence to Yuxian Xia.

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Cao, Y., Jiao, R. & Xia, Y. A strong promoter, PMagpd, provides a tool for high gene expression in entomopathogenic fungus, Metarhizium acridum . Biotechnol Lett 34, 557–562 (2012). https://doi.org/10.1007/s10529-011-0805-3

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  • DOI: https://doi.org/10.1007/s10529-011-0805-3

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