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Transformation of Metarhizium anisopliae with benomyl resistance and green fluorescent protein genes provides a tag for genetically engineered strains

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Abstract

A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the haemolymph of the infected locust, and the benomyl-resistance was maintained. Results suggested that the two markers provided a useful tool for screening and monitoring the engineered strains even after infection.

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Acknowledgements

The research was granted by the Natural Science Foundation of Chongqing Sci-tech Commission, P. R. China (NO. 8564 and 2006BB1141).

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Authors and Affiliations

  1. Genetic Engineering Research Center, Institute of Bioengineering, Chongqing University, Chongqing, 400044, P.R. China

    Yueqing Cao, Guoxiong Peng, Zhengbo He, Zhongkang Wang, Youping Yin & Yuxian Xia

  2. Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, P.R. China

    Yueqing Cao, Guoxiong Peng, Zhengbo He, Zhongkang Wang, Youping Yin & Yuxian Xia

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  1. Yueqing Cao
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  2. Guoxiong Peng
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  3. Zhengbo He
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  4. Zhongkang Wang
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  5. Youping Yin
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Correspondence to Yuxian Xia.

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Cao, Y., Peng, G., He, Z. et al. Transformation of Metarhizium anisopliae with benomyl resistance and green fluorescent protein genes provides a tag for genetically engineered strains. Biotechnol Lett 29, 907–911 (2007). https://doi.org/10.1007/s10529-007-9332-7

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  • DOI: https://doi.org/10.1007/s10529-007-9332-7

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