Abstract
A bacteriocin-producing Lactococcus lactis subsp. lactis was isolated from the intestine of olive flounder. The bacteriocin was identified as nisin Z. It was active against Gram-positive bacteria. Nisin Z at 3,200 arbitrary units (AU) was more effective in seawater than in PBS; growth of Streptococcus iniae was completely inhibited within 3 h. Nisin Z preparations with 3.5% (w/v) NaCl was the most effective against S. iniae being similar to nisin Z in seawater. Nisin Z is thus a good alternative to antibiotics to prevent streptococcosis caused by S. iniae aquaculture systems.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agnew W, Barnes AC (2007) Streptococcus iniae: an aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination. Vet Microbiol 122:1–15
Cho MY, Kim MS, Choi HS, Park GH, Kim JW, Park MS, Park MA (2008) A statistical study on infectious disease of cultured olive flounder, Paralichthys olivaceus in korea. J Fish Pathol 21:271–278
de Arauz LJ, Jozala AF, Pinheiro GS, Mazzola PG, Junior AP, Penna TCV (2008) Nisin expression production from Lactococcus lactis in milk whey medium. J Chem Technol Biotechnol 83:325–328
Desriac F, Defer D, Bourgougnon N, Brillet B, Chevalier PL, Fleury Y (2010) Bacteriocin as weapons in the marine animal-associated bacteria warfare: inventory and potential applications as an aquaculture probiotic. Mar Drugs 8:1153–1177
Eldar A, Horovicz A, Bercovier H (1997) Development and efficacy of a vaccine against streptococcus iniae infection in farmed rainbow trout. Vet Immunol Immunopath 56:175–183
Hammou FB, Skail SN, Idaomar M, Abrini J (2010) Combinations of nisin with salt (NaCl) to control Listeria monocytogenes on sheep natural sausage casings at 6°C. African J Biotechnol 9:1190–1195
Kathiresan K, Thiruneelakandan G (2008) Prospects of lactic acid bacteria of marine origin. Indian J Biotechnol 7:170–177
Li C, Jinghua B, Wei L, Zhaoling C, Fan O (2001) Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs. Biotechnol Prog 17:366–368
Lim SM, Im DS (2009) Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J Microbiol Biotechnol 19(2):178–186
Mantovani HC, Haijing H, Worobo RW, Russell JB (2002) Bovicin HC5, a bacteriocin from Streptococcus bovis HC5. Microbiology 148:3347–3352
Meghrous J, Lacroix C, Bouksaim M, Lapointe G, Simard RE (1997) Genetic and biochemical characterization of nisin Z produced by Lactococcus lactis ssp. lactis biovar. diacetylactis UL719. J Appl Microbiol 83:133–138
Moon JS, Jang H, Kim JY, Joh SJ, Kim MJ, Son SW (2007) Evaluation on efficacy of β-hemolytic Streptococcus iniae vaccine on olive flounder, Paralichthys olivaceus. J Vet Sci 47:291–298
Nes IF, Johnsborg O (2004) Exploration of antimicrobial potential in LAB by genomics. Curr Opin Biotechnol 15:100–104
Noonpakdee W, Santivarangkna C, Jumriangrit P, Sonomoto K, Panyim S (2003) Isolation of nisin-producing Lactococcus lactis WNC 20 strain from nham, a traditional Thai fermented sausage. Int J Food Microbiol 81:137–145
Olasupo NA, Schillinger U, Narbad A, Dodd H, Holzapfel WH (1999) Occurrence of nisin Z production in Lactococcus lactis BFE 1500 isolated from wara, a traditional Nigerian cheese product. Int J Food Microbiol 53:141–152
Park SH, Itoh K, Kikuchi E, Niwa H, Fujisawa T (2003) Identification and characteristics of nisin Z-producing Lactococcus lactis subsp. lactis isolated from Kimchi. Curr Microbiol 46:385–388
Pawar DD, Malik SVS, Bhilegaonkar KN, Barbuddhe SB (2000) Effect of nisin and its combination with sodium chloride on the survival of Listeria monocytogenes added to raw buffalo meat mince. Meat Sci 56:215–219
Schagger H, von Jacow G (1987) Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379
Tuncer Y, Ozden B (2010) Partial biochemical characterization of nisin-like bacteriocin produced by Lactococcus lactis subsp. lactis YBD11 isolated from Boza, a traditional fermented Turkish beverage. Romanian Biotechnol Lett 15:4940–4948
Watson AK, Kaspar H, Lategan MJ, Gibson L (2008) Probiotics in aquaculture: the need, principles and mechanisms of action and screening processes. Aquaculture 274:1–14
Wiedemann I, Breukink E, van Kraajj C, Kuipers OP, Bierbaum G, de Krujjiff B, Sahl HG (2001) Specific binding of nisin to the peptidoglycan precursor lipid II combines pore formation and inhibition of cell wall biosynthesis for potent antibiotic activity. J Biol Chem 276:1772–1779
Winkowsky K, Bruno M, Montville TJ (1994) Correlation of bioenergetic parameters with cell death in Listeria monocytogenes cells exposed to nisin. Appl Environ Microbiol 60:4186–4188
Acknowledgements
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant 2010-0006364).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Heo, WS., Kim, EY., Kim, YR. et al. Salt effect of nisin Z isolated from a marine fish on the growth inhibition of Streptococcus iniae, a pathogen of streptococcosis. Biotechnol Lett 34, 315–320 (2012). https://doi.org/10.1007/s10529-011-0766-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-011-0766-6