Abstract
A custom-made stainless steel column was designed to contain various materials that would trap the hydrocarbons and hydrocarbon derivatives during the processes of fungal fermentation ultimately yielding preparative amounts of volatile organic substances (VOCs). Trapping materials tested in the column were Carbotrap materials A and B (Supelco) as well as bentonite-shale from the oil bearing areas of Eastern Montana, the former allowed for the effective and efficient trapping of VOCs from purged cultures of Hypoxylon sp. Trapping efficiencies of various materials were measured by both gravimetric as well as proton transfer reaction mass spectroscopy with the Carbotraps A and B being 99% efficient when tested with known amounts of 1,8-cineole. Trapped fungal VOCs could effectively be removed and recovered via controlled heating of the stainless steel column followed by passage of the gases through a liquid nitrogen trap at a recovery rate of ca 65–70%. This method provides for the recovery of mg quantities of compounds normally present in the gas phase that may be needed for spectroscopy, bioassays and further separation and analysis and may have wide applicability for many other biological systems involving VOCs. Other available Carbotraps could be used for other applications.
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References
Adams NG, Richmond DM (1951) Aromatic hydrocarbons in some diesel fuel fractions. Anal Chem 23:129–133
Avci R, Hagenston AM, Equall NL, Groenewold GS, Gresham GL, Dahl DA (1999) Ion extraction from insulating fibers in ToF-SIMA. Surf Interface Anal 27:789–796
Barton A, Tjandra J (1989) Eucalyptus oil as a cosolvent in water-ethanol-gasoline mixtures. Fuel 68:11–17
Croteau R, Satterwhite DM, Wheeler CJ, Felton NM (1988) Biosynthesis of monoterpenes: stereochemistry of the enzymatic cyclization of geranyl pyrophosphate to (−)-endo-fenchol. J Biol Chem 263:15449–15453
Ezra D, Hess W, Strobel G (2004a) Unique wild type endophytic isolates of Muscodor albus, a volatile antibiotic producing fungus. Microbiology 150:4023–4031
Ezra D, Jasper J, Rogers T, Knighton B, Grimsrud E, Strobel G (2004b) Proton transfer reaction-mass spectroscopy as a technique to measure volatile emissions of Muscodor albus. Plant Sci 166:1471–1477
Fuyan L, Mingming L, Keener TC, Zifei L, Soon-Jai Khangb S (2005) The organic composition of diesel particulate matter, diesel fuel and engine oil of a non-road diesel generator. J Environ Monit 7:983–988
Griffin MA, Spakowicz DJ, Gianoulis TA, Strobel SA (2010) Volatile organic compound production by organisms in the genus Ascocoryne and a re-evaluation of myco-diesel production by NRRL 50072. Microbiology 156:3814–3829
Lindinger W, Hansel A, Jordan A (1998) On-line monitoring of volatile organic compounds at pptv levels by means of proton-transfer-reaction mass spectrometry (PTR-MS): medical applications, food control and environmental research. Int J Mass Spectrom Ion Process 173:191–241
Strobel GA, Dirksie E, Sears J, Markworth C (2001) Volatile antimicrobials from Muscodor albus, a novel endophytic fungus. Microbiology 147:2943–2950
Strobel GA, Knighton B, Kluck K, Ren Y, Livinghouse T, Griffin M, Spakowicz D, Sears J (2008) The production of myco-diesel hydrocarbons and their derivatives by the endophytic fungus Gliocladium roseum (NRRL 50072). Microbiology 154:3319–3328
Sugito K, Takeda S (1981) Patent No. 4,297,109. Japan
Tomsheck A, Strobel GA, Booth E, Geary B, Spakowicz D, Knighton B, Floerchinger C, Sears J, Liarzi O, Ezra D (2010) Hypoxylon sp. an endophyte of Persea indica, producing 1, 8-cineole and other bioactive volatiles with fuel potential. Microb Ecol 60:903–914
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The authors appreciate the financial assistance of the US-DoE.
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Booth, E., Strobel, G., Knighton, B. et al. A rapid column technique for trapping and collecting of volatile fungal hydrocarbons and hydrocarbon derivatives. Biotechnol Lett 33, 1963–1972 (2011). https://doi.org/10.1007/s10529-011-0660-2
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DOI: https://doi.org/10.1007/s10529-011-0660-2