Abstract
The maize zsS3a gene codes for starch synthase. Transcriptional analysis revealed that it is mainly expressed in endosperm and is induced by abscisic acid (ABA). The 5′-flanking region of zsS3a was isolated, and a 1772 bp zsS3a promoter (PzsS3a) was fused to a Luc reporter gene with a maize Adh1 intron. Transient expression assay by bombardment transformation showed that, although the addition of the Adh1 intron enhanced the promoter activity approx. 52-fold, it did not alter the promoter specificity. PzsS3a with the Adh1 intron drove the Luc gene preferentially and it was highly expressed in the endosperm relative to the embryo but not in the leaf or root. Furthermore, the promoter activity in the endosperm was enhanced four fold by 100 mM ABA.
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References
Ahn YO, Kim SH, Kim CY, Lee JS, Kwak SS, Lee HS (2010) Exogenous sucrose utilization and starch biosynthesis among sweet potato cultivars. Carbohydr Res 345(1):55–60
Akihiro T, Mizuno K, Fujimura T (2005) Gene expression of ADP-glucose pyrophosphorylase and starch contents in rice cultured cells are cooperatively regulated by sucrose and ABA. Plant Cell Physiol 46:937–946
Antal Z, Rascle C, Fèvre M, Bruel C (2004) Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags. Curr Genet 46:240–246
Bajaj S, Mohanty A (2005) Recent advances in rice biotechnology towards genetically superior transgenic rice. Plant Biotechnol J 3:275–307
Chen PW, Chiang CM, Tseng TH, Yu SM (2006) Interaction between rice MYBGA and the gibberellin response element controls tissue-specific sugar sensitivity of α-amylase genes. Plant Cell 18:2326–2340
Chiou CY, Wu KQ, Yeh KW (2008) Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower Ramsey. Biotechnol Lett 30:1861–1866
Cornejo MJ, Luth D, Blankenship KM, Anderson OD, Blechl AE (1993) Activity of maize ubiquitin promoter in transgenic rice. Plant Mol Biol 23:567–581
Gao M, Wanat J, Stinard PS, James MG, Myers AM (1998) Characterization of dull1, a maize gene coding for a novel starch synthase. Plant Cell 10:399–412
Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database. Nucleic Acids Res 27:297–300
Lu CA, Lim EK, Yu SM (1998) Sugar response sequence in the promoter of a rice alpha-amylase gene serves as a transcriptional enhancer. J Biol Chem 273:10120–10131
Mascarenhas D, Mettler IJ, Pierce DA, Lowe HW (1990) Intron-mediated enhancement of heterologous gene expression in maize. Plant Mol Biol 15:913–920
Muller M, Knudsen S (1993) The nitrogen response of a barley C-hordein promoter is controlled by positive and negative regulation of the GCN4 and endosperm box. Plant J 4:343–355
Qu LQ, Xing YP, Liu WX, Xu XP, Song YR (2008) Expression pattern and activity of six glutelin gene promoters in transgenic rice. J Exp Bot 59(9):2417–2424
Takaiwa F, Takagi H, Hirose S, Wakasa Y (2007) Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice. Plant Biotechnol J 5:84–92
Xie CJ, Zhang BB, Wang D, Kou F, Zhao XP, Yang XY (2011) Molecular cloning and characterization of an acheneseed-specific promoter from motherwort (Leonurus japonicus Houtt). Biotechnol Lett 33:167–172
Yang JC, Zhang JH, Wang ZQ, Zhu QS, Wang W (2001) Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol 127:315–323
Zhang JH, Sui XZ, Li B, Su BL, Li JM, Zhou DX (1998) An improved water-use efficiency for winter wheat grown under reduced irrigation. Field Crop Res 59:91–98
Acknowledgments
This work was financially supported by grants from High-tech Research Plan of China (863) (2008AA863019) and the National Major Special Project of China on New Varieties Cultivation for Transgenic Organisms (2009ZX08003-022B).
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Hu, YF., Li, Yp., Zhang, J. et al. PzsS3a, a novel endosperm specific promoter from maize (Zea mays L.) induced by ABA. Biotechnol Lett 33, 1465–1471 (2011). https://doi.org/10.1007/s10529-011-0582-z
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DOI: https://doi.org/10.1007/s10529-011-0582-z