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Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt)

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Abstract

LJAMP1 is a small antimicrobial protein purified previously from the seeds of motherwort, and it is expressed preferentially in seeds. A 794-bp upstream sequence of the ATG start codon was isolated using a genome walking method and cloned into the upstream of the β-glucuronidase (GUS) reporter gene to determine the GUS tissue-specific expression pattern. The transgenic tobacco showed that pLJAMP1 promoter derived GUS reporter gene special expression in pollen, achene and seed. The analysis of cis-acting elements also revealed pLJAMP1 promoter contained pollen and seed related transcriptional control elements.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant no. 30771388) and the Foundation for Excellent Doctor of Southwest University (kb2009018).

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Authors and Affiliations

  1. Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education) and the School of Life Science, Southwest University, 400715, Chongqing, China

    Chengjian Xie, Beibei Zhang, De Wang, Fei Kou, Xupeng Zhao & Xingyong Yang

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  1. Chengjian Xie
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  2. Beibei Zhang
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  3. De Wang
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  4. Fei Kou
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  5. Xupeng Zhao
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  6. Xingyong Yang
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Corresponding author

Correspondence to Xingyong Yang.

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Chengjian Xie, Beibei Zhang—these authors contributed equally to this work.

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Xie, C., Zhang, B., Wang, D. et al. Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt). Biotechnol Lett 33, 167–172 (2011). https://doi.org/10.1007/s10529-010-0392-8

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  • DOI: https://doi.org/10.1007/s10529-010-0392-8

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