Abstract
To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l−1 h−1, respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production.
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Acknowledgments
This work was supported in part by a grant from the Ministry of Agriculture of Korea and in part by a grant from the Korean Ministry of Education, Science, and Technology (Regional Core Research Program/Biohousing Research Institute).
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Ko, B.S., Kim, DM., Yoon, B.H. et al. Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis . Biotechnol Lett 33, 1209–1213 (2011). https://doi.org/10.1007/s10529-011-0558-z
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DOI: https://doi.org/10.1007/s10529-011-0558-z