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Engineering of a multi-functional extracellular matrix protein for immobilization to bone mineral hydroxyapatite

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Abstract

Purpose of Work

We have developed a strategy of designing multi-functional extracellular matrix proteins for functionalizing bone tissue engineering scaffolds and other biomedical surfaces to achieve improvements in bone grafting, bone repair and bone regeneration.

We developed a novel extracellular matrix protein designed to have a cell adhesive RGD sequence derived from fibronectin and active functional unit of osteocalcin (OC) containing Ca2+-binding sites for immobilization to mineral component of bone, hydroxyapatite (HA). The fusion protein, designated FNRGD/OC, was expressed in Escherichia coli and purified with affinity chromatography using a His-tag. The resultant FNRGD/OC fusion protein preferentially bound to HA, promoted cell adhesive activity, and stimulated differentiation of MC3T3-E1 cell.

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Acknowledgments

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-314-E00183), the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korea government (MOST) (R01-2007-000-20183), the Grants (#2009-0093829: priority research centers program, and #M10755080002-07N5508-00212) through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology.

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Authors and Affiliations

  1. Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712, Korea

    Wonmo Kang & Jun-Hyeog Jang

  2. Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry, Seoul, 110-749, Korea

    Tai-Il Kim

  3. Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, 330-714, Korea

    Yerang Yun & Hae-Won Kim

  4. Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714, Korea

    Hae-Won Kim

Authors
  1. Wonmo Kang
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  2. Tai-Il Kim
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  3. Yerang Yun
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  4. Hae-Won Kim
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  5. Jun-Hyeog Jang
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Corresponding author

Correspondence to Jun-Hyeog Jang.

Additional information

Wonmo Kang and Tai-Il Kim contributed equally to this work.

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Kang, W., Kim, TI., Yun, Y. et al. Engineering of a multi-functional extracellular matrix protein for immobilization to bone mineral hydroxyapatite. Biotechnol Lett 33, 199–204 (2011). https://doi.org/10.1007/s10529-010-0412-8

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  • DOI: https://doi.org/10.1007/s10529-010-0412-8

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