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Kinetics of lipase catalyzed isoamyl acetate synthesis at high hydrostatic pressure in hexane

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Abstract

Lipase-catalyzed synthesis of isoamyl acetate in hexane at 10–250 MPa at 80°C and 1–100 MPa at 40°C resulted in activation volumes of −12.9 ± 1.7 and −21.6 ± 2.9 cm3 mol−1, respectively. Increasing pressure from 10 to 200 MPa resulted in approximately 10-fold increase in V max at both 40 and 80°C. Pressure increased the K m from 2.4 ± 0.004 to 38 ± 0.78 mM at 40°C. In contrast, at 80°C the pressure did not affect the K m.

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References

  • Eisenmenger MJ, Reyes-De-Corcuera JI (2009a) High hydrostatic pressure increased stability and activity of immobilized lipase in hexane. Enzyme Microb Tech 45(2):118–125

    Article  CAS  Google Scholar 

  • Eisenmenger MJ, Reyes-De-Corcuera JI (2009b) High pressure enhancement of enzymes: a review. Enzyme Microb Tech 45(5):331–347

    Article  CAS  Google Scholar 

  • Kunugi S (1992) Enzyme reactions under high pressure and their applications. Annu NY Acad Sci 672:293–304

    Article  CAS  Google Scholar 

  • Lee LS, Lin RG (1999) Reaction and phase equilibria of esterification of isoamyl alcohol and acetic acid at 760 mmHg. Fluid Phase Equilib 165(2):261–278

    Article  CAS  Google Scholar 

  • Michels PC, Clark DS (1992) Pressure-dependence of enzyme catalysis ACS Symp Ser 498:108–121

    Article  CAS  Google Scholar 

  • Romero MD, Calvo L, Alba C, Daneshfar A, Ghaziaskar HS (2005) Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in n-hexane. Enzyme Microb Tech 37(1):42–48

    Article  CAS  Google Scholar 

  • Romero MD, Calvo L, Alba C, Daneshfar A (2007) A kinetic study of isoamyl acetate synthesis by immobilized lipase-catalyzed acetylation in n-hexane. J Biotechnol 127(2):269–277

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This project was supported by a Research Innovation Grant from the University of Florida Institute of Food and Agricultural Sciences.

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Authors and Affiliations

  1. Department of Food Science and Human Nutrition, University of Florida/IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA

    Michael J. Eisenmenger & José I. Reyes-De-Corcuera

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  1. Michael J. Eisenmenger
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  2. José I. Reyes-De-Corcuera
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Correspondence to José I. Reyes-De-Corcuera.

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Eisenmenger, M.J., Reyes-De-Corcuera, J.I. Kinetics of lipase catalyzed isoamyl acetate synthesis at high hydrostatic pressure in hexane. Biotechnol Lett 32, 1287–1291 (2010). https://doi.org/10.1007/s10529-010-0288-7

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  • DOI: https://doi.org/10.1007/s10529-010-0288-7

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