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A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana × P. bolleana)

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Abstract

The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4°C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 μM). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana × P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.

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References

  • Attila H, Sára E, Tibor J, Erika T, Gábor H, Dénes D (2004) Transgenic tobacco plants overproducing alfalfa aldose/aldehyde reductase show higher tolerance to low temperature and cadmium stress. Plant Sci 166:1329–1333

    Article  Google Scholar 

  • Basia V, Arie A (2005) Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotechnol 16:123–132

    Article  Google Scholar 

  • Breton G, Danyluk J, Charron JF, Sarhan F (2003) Expression profiling and bioinformatic analyses of a novel stress-regulated multispanning transmembrane protein family from cereals and Arabidopsis. Plant Physiol 132:64–74

    Article  PubMed  CAS  Google Scholar 

  • Chen TH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opinn Plant Biol 5:250–257

    Article  CAS  Google Scholar 

  • Ferro M, Salvi D, Brugiere S, Miras S, Kowalski S, Louwagie M, Garin J, Joyard J, Rolland N (2003) Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana. Mol Cellular Proteomics 2:325–345

    CAS  Google Scholar 

  • Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14:1675–1690

    Article  PubMed  CAS  Google Scholar 

  • Friso G, Giacomelli L, Ytterberg AJ, Peltier JB, Rudella A, Sun Q, Wijk KJ (2004) In-depth analysis of the thylakoid membrane proteome of Arabidopsis thaliana chloroplasts: new proteins, new functions, and a plastid proteome database. Plant Cell 16:478–499

    Article  PubMed  CAS  Google Scholar 

  • Froehlich JE, Wilkerson CG, Ray WK, McAndrew RS, Osteryoung KW, Gage DA, Phinney BS (2003) Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis. J Proteome Res 2:413–425

    Article  PubMed  Google Scholar 

  • Guo Y, Xiong L, Ishitani M, Zhu JK (2002) An Arabidopsis mutation in translation elongation factor 2 causes superinduction of CBF/DREB1 transcription factor genes but blocks the induction of their downstream targets under low temperatures. Proc Natl Acad Sci USA 11:7786–7791

    Article  Google Scholar 

  • He LX, Ban Y, Hiromichi I, Narumi M, Liu JH, Takaya M (2008) Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Phytochemistry 69:2133–2141

    Article  PubMed  CAS  Google Scholar 

  • Jaakola L, Pirttila AM, Halonen M, Hohtola A (2001) Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Mol Biotechnol 19:201–300

    Article  PubMed  CAS  Google Scholar 

  • Li HY, Wang YC, Jiang J, Liu GF, Gao CQ, Yang CP (2009) Identification of genes responsive to salt stress on Tamarix hispida roots. Gene (In press)

  • Machuka J, Bashiardes S, Ruben E, Spooner K, Cuming A, Knight C, Cove D (1999) Sequence analysis of expressed sequence tags from an ABA treated cDNA library identifies stress response genes in the moss Physcomitrella patens. Plant Cell Physiol 40:378–387

    PubMed  CAS  Google Scholar 

  • Malgorzata M, Posmyk Christoph B, Katarzyna S, Krystyna MJ, Franc-oise C (2005) Antioxidant enzymes and isoflavonoids in chilled soybean (Glycine max L. Merr) seedlings. J Plant Physiol 162:403–412

    Article  Google Scholar 

  • Maruyama K, Sakuma Y, Kasuga M, Ito Y, Seki M, Goda H, Shimada Y, Yoshida S, Shinozaki K, Yamaguchi-Shinozaki K (2004) Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems. Plant J 38:982–993

    Article  PubMed  CAS  Google Scholar 

  • Morsy MR, Almutairi AM, Gibbons J, Yun SJ, de Los Reyes BG (2005) The OsLti6 genes encoding low-molecular weight membrane proteins are differentially expressed in rice cultivars with contrasting sensitivity to low temperature. Gene 344:171–180

    Article  PubMed  CAS  Google Scholar 

  • Okawa K, Nakayama K, Kakizaki T, Yamashita T, Inaba T (2008) Identification and characterization of Cor413im proteins as novel components of the chloroplast inner envelope. Plant Cell Environ 31:1470–1483

    Article  PubMed  CAS  Google Scholar 

  • Quan RD, Shang M, Zhang H, Zhao YX, Zhang JR (2004) Improved chilling tolerance by transformation with betA gene for the enhancement of glycinebetaine synthesis in maize. Plant Sci 166:141–149

    Article  CAS  Google Scholar 

  • Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292

    Article  PubMed  CAS  Google Scholar 

  • Sreenivasulu N, Sopory SK, Kavi Kishor PB (2007) Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene 388:1–13

    Article  PubMed  CAS  Google Scholar 

  • Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol Biol 50:571–599

    Article  PubMed  CAS  Google Scholar 

  • Tzfira T, Jensen CS, Wang WX, Zuker A, Vinocur B, Altman A, Vainstein A (1997) Transgenic Populus tremula: a step-by-step protocol for its Agrobacterium-mediated transformation. Plant Mol Biol Rep 15:219–235

    Article  CAS  Google Scholar 

  • Wang YC, Jiang J, Zhao X, Liu GF, Yang CP, Zhan LP (2006) A novel LEA gene from Tamarix androssowii confers drought tolerance in transgenic tobacco. Plant Sci 171:655–662

    Article  CAS  Google Scholar 

  • Wang YC, Ma H, Liu GF, Xu CX, Zhang DW, Ban QY (2008) Analysis of gene expression profile of limonium bicolor under NaHCO3 stress using cDNA microarray. Plant Mol Biol Rep 26:241–254

    Article  Google Scholar 

  • Xu DQ, Huang J, Guo SQ, Yang X, Bao YM, Tang HJ, Zhang HS (2008) Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.). FEBS Lett 582:1037–1043

    Article  PubMed  CAS  Google Scholar 

  • Zhu JH, Dong CH, Zhu JK (2007) Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Curr Opin Plant Biol 10:290–295

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This study was supported by National Natural Science Foundation (Grant No. 30571509), Heilongjiang province scientific and technological project (Grant No. GB06B303 and WB07N02).

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Authors and Affiliations

  1. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, China

    Xiao-Hong Guo, Jing Jiang, Shi-Jie Lin, Bai-Chen Wang, Yu-Cheng Wang, Gui-Feng Liu & Chuan-Ping Yang

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  1. Xiao-Hong Guo
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  2. Jing Jiang
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  3. Shi-Jie Lin
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  4. Bai-Chen Wang
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  5. Yu-Cheng Wang
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  6. Gui-Feng Liu
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  7. Chuan-Ping Yang
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Correspondence to Jing Jiang.

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Guo, XH., Jiang, J., Lin, SJ. et al. A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana × P. bolleana). Biotechnol Lett 31, 1079–1087 (2009). https://doi.org/10.1007/s10529-009-9959-7

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  • DOI: https://doi.org/10.1007/s10529-009-9959-7

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