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Functional characterization of kanB by complementing in engineered Streptomyces fradiae ∆neo6::tsr

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Abstract

A putative aminotransferase gene, kanB, lies in the biosynthetic gene cluster of Streptomyces kanamyceticus ATCC 12853 and has 66% identity with neo6 in neomycin biosynthesis. Streptomyces fradiae ∆neo6::tsr was generated by disrupting neo6 in the neomycin producer Streptomyces fradiae. Neomycin production was completely abolished in the disruptant mutant but was restored through self-complementation of neo6. S. fradiae HN4 was generated through complementation with kanB in Streptomyces fradiae ∆neo6::tsr. Based on metabolite analysis by ESI/MS and LC/MS, neomycin production was restored in Streptomyces fradiae HN4. Thus, like neo6, kanB also functions as a 2-deoxy-scyllo-inosose aminotransferase that has dual functions in the formation of 2-deoxy-scyllo-inosose (DOS).

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Acknowledgments

This work was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology (Grant MG02-0301-004-2-3-1), Republic of Korea.

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Authors and Affiliations

  1. Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University, 100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, 336-708, Korea

    Hum Nath Jnawali, Bimala Subba, Kwangkyoung Liou & Jae Kyung Sohng

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  1. Hum Nath Jnawali
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  2. Bimala Subba
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  3. Kwangkyoung Liou
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  4. Jae Kyung Sohng
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Correspondence to Jae Kyung Sohng.

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Jnawali, H.N., Subba, B., Liou, K. et al. Functional characterization of kanB by complementing in engineered Streptomyces fradiae ∆neo6::tsr . Biotechnol Lett 31, 869–875 (2009). https://doi.org/10.1007/s10529-009-9937-0

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  • DOI: https://doi.org/10.1007/s10529-009-9937-0

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