Abstract
A novel OSPGYRP gene encoding a rice proline-, glycine- and tyrosine-rich protein was isolated from cold-stress treated rice seedlings using suppression subtractive hybridization. Both amino acid sequence analysis and subcellular localization confirm that OsPGYRP is a novel protein involved in vesicle trafficking. The expression of the OSPGYRP gene was induced by cold, salt, and osmotic stress. In addition, expression of the OSPGYRP gene in E. coli increased the resistance to cold stress. These results show that OsPGYRP is a novel protein involved in vesicle trafficking and plays an important role in plant adaptation to stress.
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Acknowledgements
This work was supported in part by the Hi-Tech Research and Development Program of China (Grant No. 2006AA10Z1F7), the State Key Basic Research and Development Plan of China (2007CB109005), the National Natural Science Foundation of China for Innovative Research Team (Grant No. 30521004) and the Key grant Project of the Chinese Ministry of Education (Grant No. 307018).
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Li, H., Yang, J., Wang, Y. et al. Expression of a novel OSPGYRP (rice proline-, glycine- and tyrosine-rich protein) gene, which is involved in vesicle trafficking, enhanced cold tolerance in E. coli . Biotechnol Lett 31, 905–910 (2009). https://doi.org/10.1007/s10529-009-9935-2
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DOI: https://doi.org/10.1007/s10529-009-9935-2