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Acyl migration during deacylation of phospholipids rich in docosahexaenoic acid (DHA): an enzymatic approach for evidence and study

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Abstract

Non-enzymatic acyl migration could be counter-productive for the preparation of structured phospholipids with docosahexaenoic acid (DHA) at a designated position. Therefore enzymatic approaches have been developed to investigate acyl migration. First, acyl migration from sn-2 to sn-1 position has been set into relief by a three step enzymatic method using a typo-selective lipase, a phospholipase A2 and a non-selective lipase. The effect of reaction temperature on acyl migration from sn-2 to sn-1 was monitored: lowering the reaction temperature from 40 to 30°C allowed a reduction of DHA migration rate of 40%. Secondly, acyl migration from sn-1 to sn-2 position was negligible. This last result was obtained through the study of structured phosphatidylcholine selective deacylation using a phospholipase A2.

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Acknowledgements

This work was financially supported by Laval Agglomération and the Conseil Général de la Mayenne (fellowships for M. Devos and S. Godet). The authors thank Pierre Goffi for his contribution to this work.

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Authors and Affiliations

  1. Mer, Molécules, Santé-EA 2160, Université de Nantes, 44072, Nantes cedex 03, France

    Laurent Poisson, Marie Devos, Stéphanie Godet, Françoise Ergan & Gaëlle Pencreac’h

  2. Département Génie Biologique, Institut Universitaire de Technologie de Laval, 52 rue des Drs Calmette et Guérin, BP 2045, 53020, Laval Cedex 9, France

    Laurent Poisson, Marie Devos, Stéphanie Godet, Françoise Ergan & Gaëlle Pencreac’h

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  1. Laurent Poisson
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  2. Marie Devos
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  3. Stéphanie Godet
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  4. Françoise Ergan
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  5. Gaëlle Pencreac’h
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Correspondence to Laurent Poisson.

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Poisson, L., Devos, M., Godet, S. et al. Acyl migration during deacylation of phospholipids rich in docosahexaenoic acid (DHA): an enzymatic approach for evidence and study. Biotechnol Lett 31, 743–749 (2009). https://doi.org/10.1007/s10529-009-9932-5

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  • DOI: https://doi.org/10.1007/s10529-009-9932-5

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