Abstract
We have detected PCR products from Salmonella spp. and Influenza A virus using Zn finger protein Zif268 and Sp1, respectively. Previously, we demonstrated a novel method of rapid and specific detection of PCR products from Legionella pneumophila genome using Zn finger protein Sp1. In principle, this methodology might be applied to the detection of most bacteria and viruses using various Zn finger proteins. Here, to demonstrate the wider applicability of our method, we detected PCR products from Salmonella spp. and the Influenza A virus. BLAST data indicated the Zif268 and Sp1 recognition sequence were located on the gyrB gene of Salmonella spp. and the nucleoprotein gene of Influenza A virus, respectively. The PCR products from the oligonucleotide corresponding to the gyrB gene of Salmonella spp. or the nucleoprotein gene of the Influenza A virus could be specifically detected by ELISA or fluorescence depolarization measurement using Zif268 or Sp1. These results indicate the wide applicability of our novel methodology.
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Acknowledgements
This work was partly supported by a Grant-in-Aid for the 21st Century COE “Future Nano-Materials” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. This work was also supported by project KH51043 of the Japan Health Sciences Foundation.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-009-9955-y
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Osawa, Y., Ikebukuro, K. & Sode, K. Zn finger-based direct detection system for PCR products of Salmonella spp. and the Influenza A virus. Biotechnol Lett 31, 725–733 (2009). https://doi.org/10.1007/s10529-009-9927-2
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DOI: https://doi.org/10.1007/s10529-009-9927-2