Abstract
Prefoldin is a hexameric chaperone that has been identified in eukaryotic cells and in Archaea. E. coli cells over-expressing the prefoldin gene from the hyperthermophilic, archaeum, Pyrococcus furiosus, grew well at 48°C while control cells were unable to grow above 46°C. The isolated and purified Pfu-prefoldin (specially the β subunit and the prefoldin) thermally protected the activity of lysozyme.
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Acknowledgements
This work is supported by National Basic Research Program of China (973 Program) No. 2004CB719606, 863 Program of China No. 2006AA02Z208, National Basic Research Program of China (973 Program) No. 2007CB714304, National Basic Research Program of China (973 Program) No. 2007CB707804 and the Key Project of CAS Knowledge Innovation Program KSC2-YW-G-005.
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Chen, H., Yang, L., Zhang, Y. et al. Over-expression and characterization of recombinant prefoldin from hyperthermophilic archaeum Pyrococcus furiosus in E. coli . Biotechnol Lett 32, 429–434 (2010). https://doi.org/10.1007/s10529-009-0156-5
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DOI: https://doi.org/10.1007/s10529-009-0156-5