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Overexpression of prefoldin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 endowed Escherichia coli with organic solvent tolerance

  • Biotechnologically Relevant Enzymes and Proteins
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Abstract

Prefoldin is a jellyfish-shaped hexameric chaperone that captures a protein-folding intermediate and transfers it to the group II chaperonin for correct folding. In this work, we characterized the organic solvent tolerance of Escherichia coli cells that overexpress prefoldin and group II chaperonin from a hyperthermophilic archeaum, Pyrococcus horikoshii OT3. The colony-forming efficiency of E. coli cells overexpressing prefoldin increased by 1,000-fold and decreased the accumulation of intracellular organic solvent. The effect was impaired by deletions of the region responsible for the chaperone function of prefoldin. Therefore, we concluded that prefoldin endows E. coli cells by preventing accumulation of intracellular organic solvent through its molecular chaperone activity.

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Acknowledgement

This study was partially supported by Grant in Aid for Scientific Research from the Japan Society for the Promotion of Science, No.17560688.

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Authors and Affiliations

  1. Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Mina Okochi, Kei Kanie, Masaki Kurimoto & Hiroyuki Honda

  2. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-8588, Japan

    Masafumi Yohda

  3. MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Hiroyuki Honda

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  1. Mina Okochi
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  2. Kei Kanie
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  3. Masaki Kurimoto
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  4. Masafumi Yohda
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  5. Hiroyuki Honda
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Correspondence to Mina Okochi.

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Okochi, M., Kanie, K., Kurimoto, M. et al. Overexpression of prefoldin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 endowed Escherichia coli with organic solvent tolerance. Appl Microbiol Biotechnol 79, 443–449 (2008). https://doi.org/10.1007/s00253-008-1450-1

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  • DOI: https://doi.org/10.1007/s00253-008-1450-1

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