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Immobilization of Escherichia coli cells with cis-epoxysuccinate hydrolase activity for d(−)-tartaric acid production

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Abstract

Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for d(−)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in κ-carrageenan gel. 23.6 g biomass/l and 43.4 g κ-carrageenan/l were the best immobilization conditions optimized by response surface methodology with 83% yield (114 U/g). Cell autolysis was observed after immobilization. Immobilized cells showed high pH (5–10) stability, thermal (up to 65°C) stability, conversion rate (>99.5%), enantioselectivity (ee > 99.6%), and were less affected by metal ions and surfactants compared with free cells. Conversion rate for immobilized cells preserved 93% after 10 repeated batches (5% for free cells).

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Acknowledgment

This work was funded by Hangzhou Bioking Biochemical Engineering Co., Ltd.

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Authors and Affiliations

  1. College of Life Science, Zhejiang University, Hangzhou, 310058, China

    Haifeng Pan, Zhipeng Xie, Jianguo Zhang & Yongquan Li

  2. Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou, 311106, China

    Wenna Bao & Jianguo Zhang

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  1. Haifeng Pan
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  2. Wenna Bao
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  3. Zhipeng Xie
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  4. Jianguo Zhang
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  5. Yongquan Li
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Correspondence to Zhipeng Xie or Jianguo Zhang.

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Pan, H., Bao, W., Xie, Z. et al. Immobilization of Escherichia coli cells with cis-epoxysuccinate hydrolase activity for d(−)-tartaric acid production. Biotechnol Lett 32, 235–241 (2010). https://doi.org/10.1007/s10529-009-0134-y

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  • DOI: https://doi.org/10.1007/s10529-009-0134-y

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