Skip to main content

Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate

Biotechnology Letters volume 31, Article number: 1917 (2009) Cite this article

Abstract

An extensive proteomics analysis has identified proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under oxidative stress induced by sodium orthovanadate (SOV). Measurement of total carotenoid accumulation per cell biomass showed an increase from 81 to 136 pg/cell after being exposed to 2.5 mM SOV, when compared to the control cells at day 3 of cultivation. A total of 83 proteins were differentially expressed in SOV-treated H. lacustris in comparison with control cells. They consisted of 34 down-regulated and 49 up-regulated proteins. Of these, 17 highly-expressed proteins were analyzed by MALDI-TOF-MS to identify the function of the differentially expressed proteins in response to oxidative stress in H. lacustris.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  • Bao H, Liu T, Chen X, Chen G (2008) Efficient in-gel proteolysis accelerated by infrared radiation for protein identification. J Proteome Res 7(12):5339–5344

    Article  CAS  PubMed  Google Scholar 

  • Chen WL, Harris DL, Joyce NC (2005) Effects of SOV-induced phosphatase inhibition and expression of protein tyrosine phosphatases in rat corneal endothelial cells. Exp Eye Res 81(5):570–580

    Article  CAS  PubMed  Google Scholar 

  • Eom H, Lee C-G, Jin E (2006) Gene expression profile analysis in astaxanthin-induced Haematococcus pluvialis using a cDNA microarray. Planta 223(6):1231–1242

    Article  CAS  PubMed  Google Scholar 

  • Gevaert K, Eggermont L, Demol H, Vandekerckhove J (2000) A fast and convenient MALDI-MS based proteomic approach: identification of components scaffolded by the actin cytoskeleton of activated human thrombocytes. J Biotechnol 78(3):259–269

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez-Fernandez L, Ortega-Ferrusola C, Macias-Garcia B, Salido GM, Pena FJ, Tapia JA (2009) Identification of protein tyrosine phosphatases and dual-specificity phosphatases in mammalian spermatozoa and their role in sperm motility and protein tyrosine phosphorylation. Biol Reprod

  • Kirazov EP, Weiss DG (1986) Effects of vanadate on the assembly and disassembly of purified tubulin. Cell Motil Cytoskeleton 6(3):314–323

    Article  CAS  PubMed  Google Scholar 

  • Kumarathasan P, Mohottalage S, Goegan P, Vincent R (2005) An optimized protein in-gel digest method for reliable proteome characterization by MALDI-TOF-MS analysis. Anal Biochem 346(1):85–89

    Article  CAS  PubMed  Google Scholar 

  • Lababpour A, Lee C-G (2006) Simultaneous measurement of chlorophyll and astaxanthin in Haematococcus pluvialis cells by first-order derivative ultraviolet–visible spectrophotometry. J Biosci Bioeng 101(2):104–110

    Article  CAS  PubMed  Google Scholar 

  • Lababpour A, Hong S-J, Lee C-G (2007) Haematococcus pluvialis cell-mass sensing using ultraviolet fluorescence spectroscopy. J Microbiol Biotechnol 17(12):1922–1930

    CAS  PubMed  Google Scholar 

  • Nebrich G, Herrmann M, Sagi D, Klose J, Giavalisco P (2007) High MS-compatibility of silver nitrate-stained protein spots from 2-DE gels using ZipPlates and AnchorChips for successful protein identification. Electrophoresis 28(10):1607–1614

    Article  CAS  PubMed  Google Scholar 

  • Steinbrenner J, Linden H (2001) Regulation of two carotenoid biosynthesis genes coding for phytoene synthase and carotenoid hydroxylase during stress-induced astaxanthin formation in the green alga Haematococcus pluvialis. Plant Physiol 125(2):810–817

    Article  CAS  PubMed  Google Scholar 

  • Thevis M, Ogorzalek Loo RR, Loo JA (2003) In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry. J Proteome Res 2(2):163–172

    Article  CAS  PubMed  Google Scholar 

  • Yang CG, Granite SJ, Van Eyk JE, Winslow RL (2006) MASCOT HTML and XML parser: an implementation of a novel object model for protein identification data. Proteomics 6(21):5688–5693

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors would like to gratefully thank Dr. Michael J. Hladik, Dept. of Biological Engineering, Inha University, and Dr. AlNaeeli Mawadda, Lab of Microbiology and Immunology, University of Rochester, NY, for their kindly reviewing the manuscript. This work was supported by the 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Education, Science & Technology, Republic of Korea.

Author information

Author notes

  1. Jae-Kweon Park

    Present address: Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyunggi, 420-743, Korea

Affiliations

  1. Department of Biological Engineering, Institute of Industrial Biotechnology, Inha University, In-Cheon, 402-751, Korea

    Ngoc-Phuong Tran, Jae-Kweon Park, Seong-Joo Hong & Choul-Gyun Lee

Authors
  1. Ngoc-Phuong Tran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jae-Kweon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Seong-Joo Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Choul-Gyun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Choul-Gyun Lee.

Additional information

Ngoc-Phuong Tran and Jae-Kweon Park are equally contributed to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (TIFF 1082 kb)

Supplementary material 2 (PPT 503 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tran, NP., Park, JK., Hong, SJ. et al. Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate. Biotechnol Lett 31, 1917 (2009). https://doi.org/10.1007/s10529-009-0095-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10529-009-0095-1

Keywords

  • Astaxanthin
  • Haematococcus lacustris
  • Protein tyrosine phosphatase
  • Proteomic analysis
  • Sodium orthovanadate