Abstract
An extensive proteomics analysis has identified proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under oxidative stress induced by sodium orthovanadate (SOV). Measurement of total carotenoid accumulation per cell biomass showed an increase from 81 to 136 pg/cell after being exposed to 2.5 mM SOV, when compared to the control cells at day 3 of cultivation. A total of 83 proteins were differentially expressed in SOV-treated H. lacustris in comparison with control cells. They consisted of 34 down-regulated and 49 up-regulated proteins. Of these, 17 highly-expressed proteins were analyzed by MALDI-TOF-MS to identify the function of the differentially expressed proteins in response to oxidative stress in H. lacustris.
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Bao H, Liu T, Chen X, Chen G (2008) Efficient in-gel proteolysis accelerated by infrared radiation for protein identification. J Proteome Res 7(12):5339–5344
Chen WL, Harris DL, Joyce NC (2005) Effects of SOV-induced phosphatase inhibition and expression of protein tyrosine phosphatases in rat corneal endothelial cells. Exp Eye Res 81(5):570–580
Eom H, Lee C-G, Jin E (2006) Gene expression profile analysis in astaxanthin-induced Haematococcus pluvialis using a cDNA microarray. Planta 223(6):1231–1242
Gevaert K, Eggermont L, Demol H, Vandekerckhove J (2000) A fast and convenient MALDI-MS based proteomic approach: identification of components scaffolded by the actin cytoskeleton of activated human thrombocytes. J Biotechnol 78(3):259–269
Gonzalez-Fernandez L, Ortega-Ferrusola C, Macias-Garcia B, Salido GM, Pena FJ, Tapia JA (2009) Identification of protein tyrosine phosphatases and dual-specificity phosphatases in mammalian spermatozoa and their role in sperm motility and protein tyrosine phosphorylation. Biol Reprod
Kirazov EP, Weiss DG (1986) Effects of vanadate on the assembly and disassembly of purified tubulin. Cell Motil Cytoskeleton 6(3):314–323
Kumarathasan P, Mohottalage S, Goegan P, Vincent R (2005) An optimized protein in-gel digest method for reliable proteome characterization by MALDI-TOF-MS analysis. Anal Biochem 346(1):85–89
Lababpour A, Lee C-G (2006) Simultaneous measurement of chlorophyll and astaxanthin in Haematococcus pluvialis cells by first-order derivative ultraviolet–visible spectrophotometry. J Biosci Bioeng 101(2):104–110
Lababpour A, Hong S-J, Lee C-G (2007) Haematococcus pluvialis cell-mass sensing using ultraviolet fluorescence spectroscopy. J Microbiol Biotechnol 17(12):1922–1930
Nebrich G, Herrmann M, Sagi D, Klose J, Giavalisco P (2007) High MS-compatibility of silver nitrate-stained protein spots from 2-DE gels using ZipPlates and AnchorChips for successful protein identification. Electrophoresis 28(10):1607–1614
Steinbrenner J, Linden H (2001) Regulation of two carotenoid biosynthesis genes coding for phytoene synthase and carotenoid hydroxylase during stress-induced astaxanthin formation in the green alga Haematococcus pluvialis. Plant Physiol 125(2):810–817
Thevis M, Ogorzalek Loo RR, Loo JA (2003) In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry. J Proteome Res 2(2):163–172
Yang CG, Granite SJ, Van Eyk JE, Winslow RL (2006) MASCOT HTML and XML parser: an implementation of a novel object model for protein identification data. Proteomics 6(21):5688–5693
Acknowledgements
The authors would like to gratefully thank Dr. Michael J. Hladik, Dept. of Biological Engineering, Inha University, and Dr. AlNaeeli Mawadda, Lab of Microbiology and Immunology, University of Rochester, NY, for their kindly reviewing the manuscript. This work was supported by the 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Education, Science & Technology, Republic of Korea.
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Ngoc-Phuong Tran and Jae-Kweon Park are equally contributed to this work.
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Tran, NP., Park, JK., Hong, SJ. et al. Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate. Biotechnol Lett 31, 1917–1922 (2009). https://doi.org/10.1007/s10529-009-0095-1
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DOI: https://doi.org/10.1007/s10529-009-0095-1