Abstract
Ternary systems consisting of monoterpenes (α-pinene or d-limonene), tert-butanol and water were used as reaction media to enhance the catalytic performance of laccases from various fungi sources (Trametes versicolor, T. hirsuta and Botrytis cinerea). The enzymes had improved catalytic efficiency (5- to 10-fold) in α-pinene-rich environment, while optimal reaction rates were in high-water content systems (15.5% v/v). The stability of laccases was significantly improved in monoterpene-based systems (up to 90% residual enzyme activity after 24 h at 30°C) in comparison with other non-conventional media. The results indicate that these ternary systems can increase the potential of laccases as catalysts for various oxidations.
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References
Almansa E, Kandelbauer A, Pereira L et al (2004) Influence of structure on dye degradation with laccase mediator systems. Biocatal Biotransfor 22:315–324
Baldrian P (2006) Fungal laccases: occurrence and properties. FEMS Microbiol Rev 30:215–242
Coll PM, Perez P, Villar E et al (1994) Domain structure of laccase I from the lignin degrading basidiomycete PM1 revealed by differential scanning calorimetry. Biochem Mol Biol Int 34:1091–1098
Festa G, Autore F, Fraternali F et al (2008) Development of new laccases by directed evolution: functional and computational analyses. Proteins 72:25–34
Flores C, Vidal C, Trejo-Hernández MR et al (2009) Selection of Trichoderma strains capable of increasing laccase production by Pleurotus ostreatus and Agaricus bisporus in dual cultures. J Appl Microbiol 106:249–257
Franzoi AC, Dupont J, Spinelli A et al (2009) Biosensor based on laccase and an ionic liquid for determination of rosmarinic acid in plant extracts. Talanta 77:1322–1327
Guebitz GM, Buchert J, Cavaco-Paulo A et al (2006) Advances in biotechnology for fibre processing. Biotechnol Lett 28:679–680
Khmelnitsky YL, Hilhost R, Veeger C (1988) Detergentless microemulsions as media for enzymatic reactions. Cholesterol oxidation catalyzed by cholesterol oxidase. Eur J Biochem 176:265–271
Kunamneni A, Camarero S, García-Burgos C et al (2008) Engineering and applications of fungal laccases for organic synthesis. Microb Cell Fact 7:32
Minussi RC, Pastore GM, Durán N (2007) Laccase induction in fungi and laccase/N–OH mediator systems applied in paper mill effluent. Bioresource Technol 98:158–164
Papadimitriou V, Pispas S, Syriou S et al (2008) Biocompatible microemulsions based on limonene: formulation, structure and applications. Langmuir 24:3380–3386
Riva S (2006) Laccases: blue enzymes for green chemistry. Trends Biotechnol 24:219–226
Rodakiewicz-Nowak J, Pozdnyakova NN, Turkovskaya OV (2005) Water-in-oil microemulsions as the reaction medium for the solvent-sensitive yellow laccases. Biocatal Biotransfor 23:271–279
Tavares AP, Rodriguez O, Macedo EA (2008) Ionic liquids as alternative co-solvents for laccase: Study of enzyme activity and stability. Biotechnol Bioeng 101:201–207
Tzialla AA, Kalogeris E, Gournis D et al (2008) Enhanced catalytic performance and stability of chloroperoxidase from Caldariomyces fumago in surfactant free ternary water-organic solvent systems. J Mol Catal B Enzym 51:24–35
Vulfson EN, Ahmed G, Gill I et al (1991) Alterations to the catalytic properties of polyphenoloxidase in detergentless microemulsions and ternary water-organic solvent mixtures. Biotech Lett 13:91–96
Wan YY, Du YM, Miyakoshi T (2008) Rhus laccase catalysis and product characterization of 1,2-dimethoxyphenol in organic solutions. Chin Chem Lett 19:333–336
Weihua Q, Hongzhang C (2008) An alkali-stable enzyme with laccase activity from entophytic fungus and the enzymatic modification of alkali lignin. Bioresource Technol 99:5480–5484
Zoumpanioti M, Stamatis H, Papadimitriou V et al (2006) Spectroscopic and catalytic studies of lipases in ternary hexane-1-propanol-water surfactantless microemulsion systems. Colloid Surf B 47:1–9
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This work has been funded by General Secretariat of Research and Technology of Greece in the frame of the project PENED 2003.
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Tzialla, A.A., Taha, A.A., Kalogeris, E. et al. Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems. Biotechnol Lett 31, 1451–1456 (2009). https://doi.org/10.1007/s10529-009-0014-5
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DOI: https://doi.org/10.1007/s10529-009-0014-5