Abstract
Four parameters, three hormones and sucrose, at seven concentrations, were designed for shoot proliferation of Penthorum chinense by uniform design. The obtained data were used for building two quadratic polynomial equations by partial least square to determine optimum concentrations of four factors. Experiments for verification confirmed that no significant difference existed between the predicted and the validated values in shoot number and length based on all inoculated explants.
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Acknowledgement
This work was financially assisted by Sichuan Provincial Youth Science and Technology Foundation (2005ZQ026-017) and Sichuan Provincial Key Subject Program (SZD0420), People’s Republic of China.
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Wang, LQ., Yang, J., Deng, E. et al. Optimizing the shoot proliferation protocol of Penthorum chinense by axillary buds. Biotechnol Lett 30, 2199–2203 (2008). https://doi.org/10.1007/s10529-008-9812-4
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DOI: https://doi.org/10.1007/s10529-008-9812-4