Abstract
Salicylideneamino-2-thiophenol (Sal) regulated the redox status and the expression of chemokines induced by tert-butyl hydroperoxide (t-BHP). Sal (100 μM) increased reduced/oxidized glutathione (GSH/GSSG) ratios and thiol (SH) levels by 210 and 157%, respectively, and decreased reactive oxygen species (ROS) levels by 60% in t-BHP-treated macrophages. The inductions of regulated upon activation, normal T-cell expressed and secreted (RANTES), monocyte chemoattractant protein-1 (MCP-1), interleukin-8 (IL-8) and hypoxia inducible factor-1α (HIF-1α) by t-BHP (10 μM) were decreased to 250, 80, 80 and 500% by Sal (100 μM), respectively. In the Sal signaling pathway, c-Jun N-terminal kinases (JNK), extracellular signal-regulated kinases (ERK) and p38 signaling protein modulation were decreased by 67, 69 and 119%, respectively, by Sal at 100 μM. Sal (100 μM) also altered cytosol and nuclear NF-κB protein expression by 169 and 5%, respectively. Sal also attenuated NF-κB nuclear binding activity. Sal thus has a protective effect against t-BHP-induced inflammation and that this, in part, is due to the inhibition of the production of RANTES, MCP-1, IL-8 and HIF-1α via the modulation of the NF-κB and mitogen-activiated protein kinase (MAPK) pathways.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amann R, Egger T, Schuligoi R, Heinemann A, Peskar BA (2001) Sodium salicylate enhances the expression of cyclooxygenase-2 in endotoxin-stimulated human mononuclear cells. Eur J Pharmacol 433:129–134
Bodamyali T, Stevens CR, Blake DR, Winyard PG (2000) Reactive oxygen nitrogen species and acute inflammation: a physiological process. In: Winyard PG, Blake DR, Evans CH (eds) Free radicals in inflammation. A Birkhauser book, Basel
Chae HJ, Chae SW, Reed JC, Kim HR (2004) Salicylate regulates COX-2 expression through ERK and wubsequent NF-kappaB activation in osteoblasts. Immunopharmacol Immunotoxicol 26:75–91
Darst M, Al-Hassani M, Li T, Yi Q, Travers JM, Lewis DA, Travers JB (2004) Augmentation of chemotherapy-induced cytokine production by expression of the platelet-activating factor receptor in a human epithelial carcinoma cell line. J Immunol 172:6330–6335
Dong Z, Huang C, Brown RE, Ma WY (1997) Inhibition of activator protein 1 activity and neoplastic transformation by aspirin. J Biol Chem 272:9962–9970
Kim HK, Park HR, Sul KH, Chung HY, Chung J (2006) Induction of RANTES and CCR5 through NF-kB activation via MAPK pathway in aged rat gingival tissues. Biotechnol Lett 28:17–23
Kopp E, Ghosh S (1994) Inhibition of NF-kappa B by sodium salicylate and aspirin. Science 265:956–959
Kwon KS, Chae HJ (2003) Sodium salicylate inhibits expression of COX-2 through suppression of ERK and subsequent NF-kappaB activation in rat ventricular cardiomyocytes. Arch Pharm Res 26:545–553
Leiro L, Alvarez E, Garcia D, Orallo F (2002) Resveratrol modulates rat macrophage functions. Int Immunopharmacol 2:767–774
Liu T-Z, Lee K-T, Chern C-L, Cheng J-T, Stern A, Tsai L-Y (2001) Free radical-triggered hepatic injury of experimental obstructive jaundice of rats involves overproduction of proinflammatory cytokines and enhanced activation of nuclear factor κB. Ann Clin Lab Sci 31:383–390
Roebuck KA (1999) Oxidant stress regulation of IL-8 and ICAM-1 gene expression: differential activation and binding of the transcription factors AP-1 and NF-kappaB. Int J Mol Med 4:223–230
Santangelo F (2003) Intracellular thiol concentration modulating inflammatory response: influence on the regulation of cell functions through cysteine prodrug approach. Curr Med Chem 10:2599–2610
Schwenger P, Bellosta P, Vietor I, Basilico C, Skolnik EY, Vilcek J (1997) Sodium salicylae induces apoptosis via p38 mitogen-activated protein kinase but tumor necrosis factor-induced c-Jun N-terminal kinase/stress-activated protein kinase activation. Proc Natl Acad Sci USA 94:2869–2873
Tauskela JS, Hewitt K, Kang LP, Comas T, Gendron T, Hakim A, Hogan M, Dukin J, Morley P (2000) Evaluation of glutathione-sensitive fluorescent dyes in cortical culture. Glia 30:329–341
Topper JN, Cai J, Falb D, Gimbrone MA Jr (1996) Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: COX-2, manganese superoxide dismutase, and endothelial cell NOS are selectively up-regulated by steady laminar shear stress. Proc Natl Acad Sci USA 93:10417–10422
Travers JB (1999) Oxidative stress can activate the epidermal platelet-activating factor receptor. J Invest Dermatol 112:279–283
Vittimberga FJ Jr, McDade TP, Perugini RA, Callery MP (1999) Sodium salicylate inhibits macrophage TNF-α production and alter MAPK activation. J Surg Res 84:143–149
Acknowledgement
This work was supported by grant No. RTI04-03-03 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chung, J., Lee, H.S., Chung, H.Y. et al. Salicylideneamino-2-thiophenol inhibits inflammatory mediator genes (RANTES, MCP-1, IL-8 and HIF-1α) expression induced by tert-butyl hydroperoxide via MAPK pathways in rat peritoneal macrophages. Biotechnol Lett 30, 1553–1558 (2008). https://doi.org/10.1007/s10529-008-9744-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-008-9744-z