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Mouse monoclonal antibodies in biological research: strategies for high-throughput production

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Abstract

Mouse monoclonal antibodies have become key components in basic research as well as in the clinical laboratory. Being invaluable tools in many biological assays, they continue to be the primary choice in the research field, although the conventional technology used for hybridoma generation and screening is a still lengthy, time-consuming and low-throughput process. With the advent of genetic immunisation and the application of automation and microarray to the traditional biological assays, the monoclonal antibody field has been revolutionised. Here, we will briefly review the most relevant strategies which have made the manufacture of murine monoclonal antibodies a faster and high-throughput technology.

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Acknowledgements

A special acknowledgement goes to Ario de Marco, Emanuela Signori, Joseph Spalluto and Brendan Fox for critical reading of the manuscript and to Alan Sawyer and Federico De Masi for their profound expertise on high-throughput monoclonal antibody production and microarray technology. This paper was partially supported by MIUR-FIRB 2006: RBIP0695BB.

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  1. Laboratory of Molecular Medicine and Biotechnology, CIR, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128, Rome, Italy

    Pieranna Chiarella & Vito Michele Fazio

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  1. Pieranna Chiarella
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  2. Vito Michele Fazio
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Correspondence to Pieranna Chiarella.

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Chiarella, P., Fazio, V.M. Mouse monoclonal antibodies in biological research: strategies for high-throughput production. Biotechnol Lett 30, 1303–1310 (2008). https://doi.org/10.1007/s10529-008-9706-5

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