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Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis

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Abstract

A recombinant Bacillus subtilis strain (KN25) was generated for the large-scale preparation of catalase. The B. subtilis katA gene encoding for catalase was cloned into the shuttle vector PRB374, downstream of the constitutively active vegII promoter, followed by transformation of the B. subtilis strain WB600 with the plasmid. The transformant strain, KN25 secretes high levels (3,500 U/ml) of catalase, which facilitates its purification. Three simple purification steps yielded nearly homogeneous catalase, with ∼70% recovery. The purified recombinant catalase has a specific activity of 34,600 U/mg under optimal conditions, and is more resistant to acidic conditions than bovine liver catalase.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (30572276). We thank Professor ZhongYi Yuan at Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, China, for providing the B. subtilis WB600 strain and the PRB374 plasmid.

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Authors and Affiliations

  1. Department of Drug Biosynthesis, School of Pharmacy, Fudan University, Shanghai, 200032, P.R. China

    Xunlong Shi, Meiqing Feng, Yujie Zhao & Pei Zhou

  2. Department of Pharmaceutics and Medicinal Chemistry, Thomas J. Long School of Pharmacy and Health Sciences, University of the Pacific, Stockton, CA, 95211, USA

    Xunlong Shi & Xin Guo

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  1. Xunlong Shi
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  2. Meiqing Feng
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  3. Yujie Zhao
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  4. Xin Guo
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Correspondence to Xin Guo or Pei Zhou.

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Shi, X., Feng, M., Zhao, Y. et al. Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis . Biotechnol Lett 30, 181–186 (2008). https://doi.org/10.1007/s10529-007-9510-7

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  • DOI: https://doi.org/10.1007/s10529-007-9510-7

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