Abstract
An isolate from the fecal samples of children was identified as Bifidobacterium longum. A plasmid isolated from it pBIFA24 was 4,892 bp with three open reading frames, ORFI, ORFII, and ORFIII. ORFI encoded a replication protein involved in a rolling-circle replication mechanism, and three sets of tandem repeat sequences featuring iteron structure were identified. Secondary structure prediction analysis of ORFII suggested it was a transmembrane protein. ORFIII showed high amino acid sequence identity with some mobilization proteins and contained an oriT sequence.
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This research was supported by a research grant of the Korea Science and Engineering (Project No: R01-2002-000-00365-0).
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Park, YS., Kim, KH., Park, J.H. et al. Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum . Biotechnol Lett 30, 145–151 (2008). https://doi.org/10.1007/s10529-007-9504-5
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DOI: https://doi.org/10.1007/s10529-007-9504-5