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Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate

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Abstract

The fadBA operon in the fatty acid β-oxidation pathway of P. putida KCTC1639 was blocked to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length PHA (mcl-PHA). Succinate at 150 mg l−1 stimulated cell growth and also the biosynthesis of medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA knockout mutant P. putida KCTC1639 was carried out for 60 h, in which mcl-PHA reached 8 g l−1 with a cell dry weight of 10.3 g l−1.

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Acknowledgement

This work was supported by the Basic Research Program (R01-2003-000-10742-00) of the Korea Science and Engineering Foundation.

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Authors and Affiliations

  1. Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, South Korea

    Minh Tri Vo, Kwang-Woo Lee, Tae-Kwon Kim & Yong-Hyun Lee

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  1. Minh Tri Vo
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  2. Kwang-Woo Lee
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  3. Tae-Kwon Kim
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  4. Yong-Hyun Lee
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Correspondence to Yong-Hyun Lee.

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Vo, M.T., Lee, KW., Kim, TK. et al. Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate. Biotechnol Lett 29, 1915–1920 (2007). https://doi.org/10.1007/s10529-007-9476-5

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  • DOI: https://doi.org/10.1007/s10529-007-9476-5

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