Abstract
An effective, simple, and convenient method to improve yeast’s multiple-stress tolerance, and ethanol production was developed. After an ethanologenic Saccharomyces cerevisiae strain SC521 was treated by nine cycles of freeze-thaw, a mutant FT9-11 strain with higher multiple-stress tolerance was isolated, whose viabilities under acetic acid, ethanol, freeze-thaw, H2O2, and heat-shock stresses were, respectively, 23-, 26-, 10- and 7-fold more than the parent strain at an initial value 2 × 107 c.f.u. per ml. Ethanol production of FT9-11 was similar (91.5 g ethanol l−1) to SC521 at 30°C with 200 g glucose l−1, and was better than the parent strain at 37°C (72.5 g ethanol l−1), with 300 (111 g ethanol l−1) or with 400 (85 g ethanol l−1) g glucose l−1.
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This study was supported by the National Basic Research Program of China (No 2004 CB719702) and the National Knowledge Innovation Project of the Chinese Academy of Sciences.
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Wei, P., Li, Z., Lin, Y. et al. Improvement of the multiple-stress tolerance of an ethanologenic Saccharomyces cerevisiae strain by freeze-thaw treatment. Biotechnol Lett 29, 1501–1508 (2007). https://doi.org/10.1007/s10529-007-9419-1
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DOI: https://doi.org/10.1007/s10529-007-9419-1