Abstract
A cryptic plasmid pL2 was isolated from Lactococcus lactis subsp. lactis and its complete nucleotide sequence was determined (5,299 bp, GenBank accession No. DQ917780). Its replication mode was identified as a theta-type belonging to the pAMβ1 family. Analysis of the nucleotide sequence revealed that pL2 contained a transfer origin, a replication origin, and five putative open reading frames (ORF1-5). ORF1 (386 amino acids) was homologous to replication protein RepB. The other open reading frames were cryptic. A small counter-transcript RNA (ctRNA) coding region was also observed.
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Acknowledgments
This work was supported by grants NSC 90-2214-E-036-005 and NSC 91-2214-E-036-001 from the National Science Council, Republic of China.
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Chang, SM., Yan, TR. DNA sequence analysis of a cryptic plasmid pL2 from Lactococcus lactis subsp. lactis . Biotechnol Lett 29, 1519–1527 (2007). https://doi.org/10.1007/s10529-007-9418-2
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DOI: https://doi.org/10.1007/s10529-007-9418-2