Abstract
We sequenced and described two cryptic plasmids from Lactiplantibacillus plantarum strain WP72/27, termed pLP2.5-11 (OP831909) and pLP3.0-4 (OP831910). Nucleotide sequencing gave the sizes of pLP2.5-11 and pLP3.0-4 as 2754 and 3197 base pairs, with G + C contents 38.89% and 40.88% and predicted two and eight putative open reading frames, respectively. The RepA protein of pLP2.5-11 shared a 99% identity with pC30il, pLP1 and pC30il, whereas the RepB protein of pLP3.0-4 shared a 98% identity with pXY3, a member of the rolling-circle replication (RCR) pC194 family. The origin of plasmid replication was predicted to consist of inverted and directed repeat sequences upstream of the Rep genes. Sequence analysis predicted that both pLP2.5-11 and pLP3.0-4 plasmids replicate via a rolling-circle process.
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This work was supported by Thaksin University, Thailand Science Research and Innovation (TSRI), and Research and Innovation Fund (NSRF).
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Accession numbers: OP035899 is accession number of 16S rRNA gene of Lactiplantibacillus plantarum strain WP72/27. OP831909 is accession number of plasmid pLP2.5-11 sequences. OP831910 is accession number of plasmid pLP3.0-4 sequences.
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Nitipan, S., Saithong, P. Characterization and sequencing analysis of pLP2.5-11 and pLP3.0-4 novel cryptic plasmids from Lactiplantibacillus plantarum WP72/27. 3 Biotech 13, 263 (2023). https://doi.org/10.1007/s13205-023-03684-y
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DOI: https://doi.org/10.1007/s13205-023-03684-y