Abstract
A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the β-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.
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This work was supported by the Korea Science Foundation Grant funded by the Korean Government (2004-F00006).
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Kim, SS., Shin, DI. & Park, HS. Transient β-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation. Biotechnol Lett 29, 965–969 (2007). https://doi.org/10.1007/s10529-007-9326-5
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DOI: https://doi.org/10.1007/s10529-007-9326-5