Abstract
α-Chymotrypsin and lysozyme were solubilized in a water/O-[(2-tridecyl, 2-ethyl-1,3-dioxolan-4-yl)methoxy]–O′-methoxy poly(ethylene glycol) (CK-2,13 surfactant)/isooctane water-in-oil microemulsion solution at 1.5–2 and 10 g l−1 for 0.15 and 1.2 M CK-2,13, respectively. Upon contact with an equal volume of 0.1 M NaH2PO4/Na2HPO4 buffer, pH 5, a three-phase system (Winsor-III system) was formed, consisting of a surfactant-rich middle phase and aqueous and isooctane-rich “excess” phases. Both enzymes were rapidly released into the aqueous excess phase, with 70% recovery of each in 30 and 60 min for microemulsion solutions containing 0.15 and 1.2 M surfactant, respectively. The recovered enzymes retained >90% of their original specific activity.
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Acknowledgments
Financial support by Nektar, Inc. (Formerly, Shearwater, Inc., Huntsville, AL) and the Alabama Department of Economic and Community Affairs, Project 1AR99-09, is gratefully acknowledged.
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Rairkar, M.E., Hayes, D.G. & Harris, J.M. Solubilization of enzymes in water-in-oil microemulsions and their rapid and efficient release through use of a pH-degradable surfactant. Biotechnol Lett 29, 767–771 (2007). https://doi.org/10.1007/s10529-006-9292-3
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DOI: https://doi.org/10.1007/s10529-006-9292-3