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A simple, rapid and effective method for total RNA extraction from Lentinula edodes

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Abstract

A rapid, inexpensive and reliable method for total RNA extraction from fruiting bodies of Lentinula edodes containing large quantities of polysaccharides and secondary metabolites is described. An initial extraction step using saturated NaCl solution facilitates the separation of nucleic acids from contaminants and, after further extraction with organic solvents and precipitation with 2-propanol, total RNA of high purity and suitable for applications such as cDNA synthesis, RT-PCR and Northern blot hybridization was obtained. The procedure may also have wider applicability for total RNA extraction from the tissues of other mushrooms.

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Acknowledgement

We thank Dr. John Buswell for linguistic revision of the manuscript.

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, P. R. China

    Jun-hui Li & Ming-jie Chen

  2. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, P. R. China

    Jun-hui Li, Chuan-hong Tang, Chun-yan Song & Zhi-yong Feng

  3. College of Food Science, Shanghai Fisheries University, Shanghai, 200090, P. R. China

    Ying-jie Pan

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  1. Jun-hui Li
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  2. Chuan-hong Tang
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  3. Chun-yan Song
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  4. Ming-jie Chen
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  5. Zhi-yong Feng
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Correspondence to Zhi-yong Feng.

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Li, Jh., Tang, Ch., Song, Cy. et al. A simple, rapid and effective method for total RNA extraction from Lentinula edodes . Biotechnol Lett 28, 1193–1197 (2006). https://doi.org/10.1007/s10529-006-9074-y

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  • DOI: https://doi.org/10.1007/s10529-006-9074-y

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