Abstract
Addition of 20 μM salicylic acid to Saussurea medusa cell cultures at day 6 resulted in jaceosidin and syringin productions up to 95 mg l−1 and 631 mg l−1 which were, respectively, about 2.5- and 2.7-fold higher than in the control. The biomass was increased from 8 to 12 g l−1. Expression of chalcone synthase gene (chs) increased sharply after 12 h treatment and was sustained up to 48 h; chalcone isomerase gene (chi) expression reached a peak at 24 h and decreased after 48 h; and phenylalanine ammonia-lyase activity increased by 7.5-fold (96 U mg−1 protein) higher than in the control after 24 h. These results indicate that salicylic acid enhances the production of jaceosidin and syringin which is accompanied by induction of the related phenylpropanoid biosynthetic enzymes.
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Acknowledgments
We thank Prof. Zhong-Jian Jia (Lanzhou University, Lanzhou city, Gansu province, China) and Prof. Ming-Yan Chen (Jilin entry-exit inspection and quarantine bureau, Changchun, China) for jaceosidin and syringin standard samples. The research was supported by the National Science Foundation of China (No. 30472158).
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Yu, ZZ., Fu, CX., Han, YS. et al. Salicylic Acid Enhances Jaceosidin and Syringin Production in Cell Cultures of Saussurea medusa . Biotechnol Lett 28, 1027–1031 (2006). https://doi.org/10.1007/s10529-006-9035-5
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DOI: https://doi.org/10.1007/s10529-006-9035-5