Abstract
A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of γ-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred tank bioreactor or shake flask). A three-fold increase in γ-decalactone productivity was obtained by increasing oscillatory mixing intensity from Reo ~482 to Reo ~1447. This was presumably related to the effective contribution of the reactor geometry to enhanced mass transfer rates between the two immiscible liquid phases involved in the process by increasing the interfacial area.
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M Aguedo N Gomes E Escamilla Garcia Y Waché M Mota JA Teixeira I Belo (2005) ArticleTitleDecalactone production by Yarrowia lipolytica under increased O2 transfer rates Biotechnol. Lett. 27 1617–1621 Occurrence Handle1:CAS:528:DC%2BD2MXhtFersLjP Occurrence Handle16245183
M Aguedo Y Wache F Coste F Husson JM Belin (2004) ArticleTitleImpact of surfactants on the biotransformation of methyl ricinoleate into gamma-decalactone by Yarrowia lipolytica J. Mol. Catal. B-Enzym. 29 31–36 Occurrence Handle1:CAS:528:DC%2BD2cXktVCrsrw%3D
S Dilsen W Paul D Herforth A Sandgathe J Altenbach-Rehm R Freudl C Wandrey D Weuster-Botz (2001) ArticleTitleEvaluation of parallel operated small-scale bubble columns for microbial process development using Staphylococcus carnosus J. Biotechnol. 88 77–84 Occurrence Handle10.1016/S0168-1656(01)00265-6 Occurrence Handle1:CAS:528:DC%2BD3MXjslSlsL8%3D Occurrence Handle11377767
P Fickers PH Benetti Y Wache A Marty S Mauersberger MS Smit JM Nicaud (2005) ArticleTitleHydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications FEMS Yeast Res. 5 527–543 Occurrence Handle1:CAS:528:DC%2BD2MXisVChu7w%3D Occurrence Handle15780653
P Girard M Jordan M Tsao FM Wurm (2001) ArticleTitleSmall-scale bioreactor system for process development and optimization Biochem. Eng. J. 7 117–119 Occurrence Handle1:CAS:528:DC%2BD3MXit1Citrc%3D Occurrence Handle11173299
Y Kostov P Harms L Randers-Eichhorn G Rao (2001) ArticleTitleLow-cost microbioreactor for high-throughput bioprocessing Biotechnol. Bioeng. 72 346–352 Occurrence Handle10.1002/1097-0290(20010205)72:3<346::AID-BIT12>3.0.CO;2-X Occurrence Handle1:CAS:528:DC%2BD3MXlvFCmsQ%3D%3D Occurrence Handle11135205
S Kumar C Wittmann E Heinzle (2004) ArticleTitleMinibioreactors Biotechnol. Lett. 26 1–10 Occurrence Handle1:CAS:528:DC%2BD3sXpvVKrtb4%3D Occurrence Handle15005143
GJ Lye P Ayazi-Shamlou F Baganz PA Dalby JM Woodley (2003) ArticleTitleAccelerated design of bioconversion processes using automated microscale processing techniques Trends Biotechnol. 21 29–37 Occurrence Handle10.1016/S0167-7799(02)00011-2 Occurrence Handle1:CAS:528:DC%2BD38XptlWjtLw%3D Occurrence Handle12480348
MR Mackley (1991) ArticleTitleProcess innovation using oscillatory flow within baffled tubes Chem. Eng. Res. Des. 69 197–199 Occurrence Handle1:CAS:528:DyaK3MXkvFGhs74%3D
X Ni S Gao (1996) ArticleTitleScale-up correlation for mass transfer coefficients in pulsed baffled reactors Chem. Eng. J. 63 157–166 Occurrence Handle1:CAS:528:DyaK28XmtlehsLo%3D
X Ni Y Zhang I Mustafa (1999) ArticleTitleCorrelation of polymer particle size with droplet size in suspension polymerisation of methylmethacrylate in a batch oscillatory-baffled reactor Chem. Eng. Sci. 54 841–850 Occurrence Handle1:CAS:528:DyaK1MXhvVyhtr4%3D
XW Ni YS Gelicourt Particlede MHI Baird NVR Rao (2001) ArticleTitleScale-up of single phase axial dispersion coefficients in batch and continuous oscillatory baffled tubes Can. J. Chem. Eng. 79 444–448 Occurrence Handle1:CAS:528:DC%2BD3MXlsFKqtLc%3D
R Puskeiler A Kusterer GT John D Weuster-Botz (2005) ArticleTitleMiniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coli Biotechnol. Appl. Biochem. 42 227–235 Occurrence Handle1:CAS:528:DC%2BD2MXht1CrtLvO Occurrence Handle15853771
N Reis AP Harvey AA Vicente JA Teixeira MR Mackley (2005) ArticleTitleFluid mechanics and design aspects of a novel oscillatory flow meso-reactor Chem. Eng. Res. Des. 83 357–371 Occurrence Handle10.1205/cherd.03401 Occurrence Handle1:CAS:528:DC%2BD2MXktFegs7o%3D
Reis N, Vicente AA, Teixeira JA, Mackley MR (2004a) Enhanced mass transfer rates of a novel oscillatory flow screening reactor. In: Summaries of the 16th International Congress of Chemical and Process Engineering, 22–26 August, Praha, Czech Republic, pp. 898–899. ISBN 80-86059-40-5
N Reis AA Vicente JA Teixeira MR Mackley (2004b) ArticleTitleResidence times and mixing of a novel continuous oscillatory flow screening reactor Chem. Eng. Sci. 59 4967–4974 Occurrence Handle1:CAS:528:DC%2BD2cXhtVCqt77I
I Walther BH Vanderschoot S Jeanneret P Arquint NF Derooij V Gass B Bechler G Lorenzi A Cogoli (1994) ArticleTitleDevelopment of a miniature bioreactor for continuous-culture in a space laboratory J. Biotechnol. 38 21–32 Occurrence Handle10.1016/0168-1656(94)90144-9 Occurrence Handle1:CAS:528:DyaK2MXitlaisbc%3D Occurrence Handle7765579
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Revisions requested 11 October 2005; Revisions received 4 January 2006
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Reis, N., Gonçalves, C.N., Aguedo, M. et al. Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium. Biotechnol Lett 28, 485–490 (2006). https://doi.org/10.1007/s10529-006-0003-x
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DOI: https://doi.org/10.1007/s10529-006-0003-x