Abstract
Two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. The first method, optimised by a factorial design, gave an 80% CGTase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mM α-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72% CGTase recovery in a two-step procedure; CGTase was obtained in the bottom phase with a purification factor of 37.
Similar content being viewed by others
References
L Bovetto D Backer J Villette P Sicard S Bouquelet (1992) ArticleTitleCyclomaltodextrin glucanotransferase from Bacillus circulans E 192 Biotechnol. Appl. Biochem 15 48–58 Occurrence Handle1532314
S Ferrarotti A Rosso M Marechal N Krymkiewicz L Marechal (1996) ArticleTitleIsolation of two strains (S-R type) of Bacillus circulans and purification of a cyclomaltodextrin-glucanotransferase Cell. Molec. Biol 42 653–657
BN Gawande AY Patkar (2001) ArticleTitlePurification and properties of a novel raw starch degrading-cyclodextrin glycosyltransferase from Klebsiella pneumoniae As−22 Enzyme Microb. Technol 28 735–743 Occurrence Handle10.1016/S0141-0229(01)00347-7 Occurrence Handle11397453
A Goel S Nene (1995) ArticleTitleModifications in the phenolphtalein method for spectrophotometric estimation of beta cyclodextrin Starch 47 399–400
M Kennedy D Krouse (1999) ArticleTitleStrategies for improving fermentation medium performance: A review J. Ind. Microbiol. Biotechnol 23 456–475 Occurrence Handle10.1038/sj.jim.2900755
KH Kim HG Lim JH Seo (1993) ArticleTitleProduction of beta-cyclodextrin from starch by cyclodextrin glycosyltransferase from alkalophilic Bacillus sp E1. Kor. J. Food Sci. Technol 25 608–610
T Kometani Y Terada T Nishimura H Takii S Okada (1994) ArticleTitlePurification and characterization of cyclodextrin glucanotransferase from an alkalophilic Bacillus species and transglycosylation at alkaline pHs Biosci. Biotech. Biochem 58 517–520
MR Kula (1990) ArticleTitleTrends and future of aqueous two-phase extraction Bioseparation 1 181–189 Occurrence Handle1368168
RF Martins R Hatti-Kaul (2002) ArticleTitleA new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: purification and characterization Enzyme Microb. Technol 30 116–124 Occurrence Handle10.1016/S0141-0229(01)00461-6
P Pongsawasdi M Yagisawa (1988) ArticleTitlePurification and some properties of cyclomaltodextrin glucanotransferase from Bacillus circulans Agric. Biol. Chem 52 1099–1103
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rosso, A., Ferrarotti, S., Miranda, M. et al. Rapid Affinity Purification Processes for Cyclodextrin Glycosyltransferase from Bacillus circulans. Biotechnol Lett 27, 1171–1175 (2005). https://doi.org/10.1007/s10529-005-8654-6
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-8654-6