Abstract
Fed-batch cultures of recombinant Escherichia coli BL21 for producing human-like collagen were performed at different specific growth rates (0.1~0.25 h−1) before induction and at a constant value of 0.05 h−1 after induction by the method of pseudo-exponential feeding. Although the final biomass (around 69 g l−1) was almost the same in all fed-batch cultures, the highest product concentration (13.6 g l−1) was achieved at the specific growth rate of 0.15 h−1 and the lowest (9.6 g l−1) at 0.25 h−1. The mean productivity of human-like collagen was the highest at 0.15 h−1 (0.57 g l−1 h−1) and the lowest at 0.1 h−1 (0.35 g l−1 h−1). In the phase before induction, the cell yield coefficient (Y X/S ) decreased when the specific growth rate increased, while the formation of acetic acid increased upto 2.5 g l−1 at 0.25 h−1. The mean product yield coefficient (Y P/S ) also decreased with specific growth rate increasing. The respiration quotient (RQ) increased slightly with specific growth rate increasing before induction, and the mean value of RQ was around 72%. The optimum growth rate for human-like collagen production was 0.15~0.2 h−1.
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Fan, D.D., Luo, Y., Mi, Y. et al. Characteristics of fed-batch cultures of recombinant Escherichia coli containing human-like collagen cDNA at different specific growth rates. Biotechnol Lett 27, 865–870 (2005). https://doi.org/10.1007/s10529-005-6720-8
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DOI: https://doi.org/10.1007/s10529-005-6720-8