Abstract
The effect of mechanical stresses generated by an extreme agitation intensity or a high aeration rate on growth parameters and cell physiology were studied during continuous cultivation of the Gram-positive bacterium Corynebacterium glutamicum. It is concluded that variations in agitation, aeration rate, or dO2 concentrations down to about 1% of saturation do not damage the bacterial cells or cause a significant change in physiological response, as measured by flow cytometry, even though the cell size was slightly reduced.
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Abbreviations
- D i :
-
dilution rate (h-1)
- D :
-
impeller diameter (m)
- F :
-
feed rate (m3/h)
- N :
-
impeller speed (rps except where stated)
- n :
-
number of impellers
- P :
-
power (W)
- Po g :
-
aerated power number (dimensionless)
- V :
-
volume of culture (m3)
- ρ:
-
density of culture (kg/m3)
- λK:
-
Kolmogoroff’s microscale of turbulence (m)
- ɛT :
-
local specific energy dissipation rate (W/kg)
- ¯ɛ T : :
-
mean specific energy dissipation rate (W/kg)
- μ:
-
dynamic viscosity (kg/m s)
- υ:
-
kinematic viscosity (m2/s).
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Chamsartra, S., Hewitt, C.J. & Nienow, A.W. The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor. Biotechnol Lett 27, 693–700 (2005). https://doi.org/10.1007/s10529-005-4690-5
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DOI: https://doi.org/10.1007/s10529-005-4690-5