Abstract
Streptomyces luteogriseus strain 099, producing a new type of macrolide antibiotic with anti-coxB6 virus and anti-HIV protease activities, was isolated from soil. PCR was optimized to amplify β-ketoacyl-ACP synthase (KS) genes. The system was optimized around the use of higher concentrations of DMSO (15% vs. 10% v/v) and dNTP (500 μM vs. 50–200 μM) and a lower annealing temperature (55 °C vs. 60–70 °C) than the normal PCR method used to amplify high GC content DNA.
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Yu, FM., Jiang, X., Wu, JC. et al. A Modified PCR System for Amplifying β-ketoacyl-ACP Synthase Gene Fragments with DNA from Streptomyces luteogriseus. Biotechnol Lett 27, 1277–1282 (2005). https://doi.org/10.1007/s10529-005-3219-2
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DOI: https://doi.org/10.1007/s10529-005-3219-2