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Comprehensive Evolution and Expression anaLysis of PHOSPHATE 1 Gene Family in Allotetraploid Brassica napus and Its Diploid Ancestors

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Abstract

The members of PHOSPHATE 1 (PHO1) family play important roles in plant phosphate (Pi) transport and adaptation to Pi deficiency. The functions of PHO1 family proteins have been reported in several plant species, with the exception of Brassica species. Here, we identified 23, 23, and 44 putative PHO1 family genes in Brassica rapa, Brassica oleracea, and Brassica napus by whole genome analysis, respectively. The phylogenetic analysis divided PHO1 family proteins into eight groups, which represented the orthologous relationships among PHO1 members. The gene structure and the conserved motif analysis indicated that the most PHO1 family genes had similar gene structures and the PHO1 proteins shared mutual conserved motifs. The chromosome distribution analysis showed that the majority of BnPHO1 family genes distributed analogously at chromosomes with BrPHO1 and BoPHO1 family genes. The data showed that PHO1 family genes were highly conserved during evolution from diploid to tetraploid. Furthermore, the expression analysis showed that PHO1 family genes had different expression patterns in plant tissues, suggesting the diversity of gene functions in Brassica species. Meanwhile, the expression analysis also revealed that some PHO1 family genes were significantly responsive to Pi deficiency, suggesting that PHO1 family genes play critical roles in Pi uptake and homeostasis under low Pi stress. Altogether, the characteristics of PHO1 family genes provide a reliable groundwork for further dissecting their functions in Brassica species.

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Funding

This work was supported by the National Natural Sciences Foundation of China (Grant No. 31971814, 31571572) and the Project of Wuhan Knowledge Innovation (Grant No. 2022020801010275).

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Author notes

  1. Jian-Feng Zhang and Hui-Hui Chu have contributed equally to this article.

Authors and Affiliations

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China

    Jian-Feng Zhang, Hui-Hui Chu, Dan Liao, Yi-Kai Tong, Ying-Ying Liu & Feng Ren

  2. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, 430074, China

    Guang-Jing Ma

  3. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, China

    Jun Li

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  1. Jian-Feng Zhang
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Contributions

JFZ, HHC, and FR designed the research; JFZ, HHC, DL, GJM, and YYL performed the experiments; JFZ, YKT, and JL analyzed the data; JFZ, HHC, and FR wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Feng Ren.

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Zhang, JF., Chu, HH., Liao, D. et al. Comprehensive Evolution and Expression anaLysis of PHOSPHATE 1 Gene Family in Allotetraploid Brassica napus and Its Diploid Ancestors. Biochem Genet 61, 2330–2347 (2023). https://doi.org/10.1007/s10528-023-10375-z

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