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Genetic Diversity of Four Populations of Silver Carp (Hypophthalmichthys molitrix) Based on Mitochondrial Sequences

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Abstract

Three mitochondrial DNA sequences (COI, ATP 8&6, and D-loop) were employed to assess the genetic diversity of four populations of silver carp from three main drainages in China, including the Yangtze River, the Amur River, and the Pearl River. As a result, 98 haplotypes were identified in combined sequences of COI, ATP8&6, and D-loop. A total of 196 variable sites and 116 parsimony-informative sites were observed. AMOVA based on assembled sequences indicated that 12.12% of the variation was among populations, while 87.88% of the variation was within populations. Additionally, the phylogenetic relationships of populations were depicted in a phylogenetic tree based on assembled sequences. Mismatch distribution analysis and the negative significant Fu’s Fs values supported population expansion in all populations. Despite the high level of genetic diversity, the establishment of a state-level original breeding farm in the Amur River basin and the Pearl River basin may be an effective conservation strategy for the protection of local unique haplotypes.

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Data Availability

All the data generated in the present research is contained in this manuscript. All of the haplotypes were submitted to GenBank, with accession numbers MT852969-MT853034 for COI, MT834756-MT834798for ATP8&6, and MT834799-MT834846 for D-loop.

Code Availability

Not applicable.

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Funding

This work was supported by the Open Project of the National and Local Joint Engineering Laboratory for Freshwater Fish Breeding (HSY2020K1) and National Freshwater Aquatic Germplasm Resource Center.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China

    Xiaochen Zhu & Yingying Zhao

  2. College of Science and Engineering, Flinders University, Adelaide, SA, 5042, Australia

    Xiaochen Zhu

  3. National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China

    Yingying Zhao, Xianhu Zheng & Xiaowen Sun

Authors
  1. Xiaochen Zhu
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  2. Yingying Zhao
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  3. Xianhu Zheng
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  4. Xiaowen Sun
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Contributions

ZY and SX conceived and designed the experiments. ZY performed the experiments. ZX collected the samples. ZX performed the data analyses. ZY and ZX wrote the manuscript. ZX revised the manuscript.

Corresponding author

Correspondence to Yingying Zhao.

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The authors declare that they have no competing interests.

Ethical Approval

This study was approved by the Animal Care and Use Committee of Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences (ACUC201711). All efforts were made to minimize the suffering of the animals.

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Zhu, X., Zhao, Y., Zheng, X. et al. Genetic Diversity of Four Populations of Silver Carp (Hypophthalmichthys molitrix) Based on Mitochondrial Sequences. Biochem Genet 61, 1231–1241 (2023). https://doi.org/10.1007/s10528-022-10314-4

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  • DOI: https://doi.org/10.1007/s10528-022-10314-4

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