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SOX2-Induced Linc-ROR Upregulation Inhibits Gastric Carcinoma Cell Proliferation and Metastasis Via the miR-580-3p/ANXA10 Pathway

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Abstract

Previous studies have proven that long intergenic non-coding RNA regulator of reprogramming (Linc-ROR) plays opposing roles in different cancer types. This work intended to investigate its functions and underlying mechanisms in gastric carcinoma (GCa) progression. RT-qPCR was utilized for gene expression measurement. GCa cell viability, apoptosis, migration, and invasion were detected by functional assays, including CCK-8, flow cytometry, and Transwell assays. ChIP assay and Dual-luciferase reporter assay were utilized to affirm the associations between genes. Linc-ROR expression dramatically declined in GCa tissues and cell lines. Linc-ROR upregulation suppressed GCa cell proliferation, migration, and invasion but accelerated GCa cell apoptosis. As for Linc-ROR-associated molecular mechanisms in GCa, SOX2 associated with Linc-ROR promoter region to activate Linc-ROR transcription in GCa cells; Linc-ROR upregulated ANXA10 level in GCa cells by competitively binding to miR-580-3p. As revealed by rescue assays, Linc-ROR-induced inhibition on malignant biological behaviors of GCa cells could be partially abated by ANXA10 deletion or miR-580-3p upregulation. SOX2-activated Linc-ROR serves as a cancer suppressor to restrain GCa progression in vitro via the miR-580-3p/ANXA10 pathway, suggesting a promising diagnostic and therapeutic target for GCa patients.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by Xiamen Medical and Health Guidance Project (No.3502Z20209031).

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Author notes

  1. Liping Bai and Yifan Zhuang have contributed equally to this work and should both be considered first authors.

Authors and Affiliations

  1. Department of Gastrointestinal Surgery, School of Medicine, Zhongshan Hospital of Xiamen University, No.201-209 Hubin South Road, Siming District, Xiamen, Fujian, China

    Liping Bai, Yifan Zhuang, Jun Xie, Kai Liu, Shengchang Yin & Feng Yan

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  1. Liping Bai
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  2. Yifan Zhuang
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  3. Jun Xie
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  4. Kai Liu
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  5. Shengchang Yin
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  6. Feng Yan
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Contributions

LB, YZ, and FY designed the present study. JX and KL performed the experiments. KL and SY analyzed the data and prepared the figures. LB, YZ, and FY drafted the initial manuscript and revised the manuscript.

Corresponding author

Correspondence to Feng Yan.

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The authors declare that they have no competing interests.

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The present study was approved by the Ethics Committee of Zhongshan Hospital of Xiamen University.

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Bai, L., Zhuang, Y., Xie, J. et al. SOX2-Induced Linc-ROR Upregulation Inhibits Gastric Carcinoma Cell Proliferation and Metastasis Via the miR-580-3p/ANXA10 Pathway. Biochem Genet 61, 1113–1127 (2023). https://doi.org/10.1007/s10528-022-10300-w

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  • DOI: https://doi.org/10.1007/s10528-022-10300-w

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