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Molecular Evaluation of Genetic Diversity in Wild-Type Mastic Tree (Pistacia lentiscus L.)

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Abstract

In this study, the patterns of genetic variation and phylogenetic relationships of mastic tree (Pistacia lentiscus L.) genotypes including 12 males and 12 females were evaluated using SSR, RAPD, ISSR, and ITS markers yielding 40, 703, 929 alleles, and 260–292 base pairs for ITS1 region, respectively. The average number of alleles produced from SSR, RAPD, and ISSR primers were 5.7, 14, and 18, respectively. The grouping pattern obtained from Bayesian clustering method based on each marker dataset was produced. Principal component analyses (PCA) of molecular data was investigated and neighbor joining dendrograms were subsequently created. Overall, the results indicated that ISSR and RAPD markers were the most powerful to differentiate the genotypes in comparison with other types of molecular markers used in this study. The ISSR results indicated that male and female genotypes were distinctly separated from each other. In this frame, M9 (Alaçatı) and M10 (Mesta Sakız Adası-Chios) were the closest genotypes and while F11 (Seferihisar) and F12 (Bornova/Gökdere) genotypes fall into same cluster and showing closer genetic relation. The RAPD pattern indicated that M8 (Urla) and M10 (Mesta Sakız Adası-Chios), and F10 (Mesta Sakız Adası-Chios) and F11 (Seferihisar) genotypes were the closest male and female genotypes, respectively.

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Acknowledgments

This work was supported by the Scientific and Technological Research Council of Turkey KBAG (Project No: 110T941) and Marmara University Research Foundation (Project No: FEN-C-YLP-090414-0099).

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Authors and Affiliations

  1. Department of Bioengineering, Faculty of Engineering, Marmara University, 34722, Istanbul, Turkey

    Alimu Abuduli & Ahu Altinkut Uncuoglu

  2. Department of Biology, Faculty of Arts and Sciences, Marmara University, 34722, Istanbul, Turkey

    Yıldız Aydin

  3. Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, 36100, Kars, Turkey

    Muhammet Sakiroglu

  4. Department of Biology, Faculty of Science, University of Dicle, Diyarbakir, 21280, Turkey

    Ahmet Onay

  5. Department of Horticulture, Agricultural Faculty, Ataturk University, 25240, Erzurum, Turkey

    Sezai Ercisli

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  1. Alimu Abuduli
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  2. Yıldız Aydin
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  3. Muhammet Sakiroglu
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  4. Ahmet Onay
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  5. Sezai Ercisli
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  6. Ahu Altinkut Uncuoglu
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Correspondence to Ahu Altinkut Uncuoglu.

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Abuduli, A., Aydin, Y., Sakiroglu, M. et al. Molecular Evaluation of Genetic Diversity in Wild-Type Mastic Tree (Pistacia lentiscus L.). Biochem Genet 54, 619–635 (2016). https://doi.org/10.1007/s10528-016-9742-0

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  • DOI: https://doi.org/10.1007/s10528-016-9742-0

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