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Three Novel SNPs in the Coding Region of the Bovine MC3R Gene and Their Associations with Growth Traits

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Abstract

The involvement of melanocortin-3 receptor (MC3R) is well recognized in the regulation of feeding efficiency, body weight, and energy homeostasis. The objective of this study was to investigate the associations between MC3R gene polymorphisms and growth traits. Three novel SNPs (c.24C→T, c.220T→A, c.734G→C) and five haplotypes were identified in 234 Xiangxi cattle. The associations between MC3R gene polymorphisms and growth traits indicated that the individuals with TT and AT genotypes maintained higher body weight than those with the AA genotype at the c.220T→A locus (P < 0.05). The animals with GG and CG genotypes had higher heart girth and body weight than those with the CC genotype at c.734G→C (P < 0.05). The animals with H3H3 and H2H3 haplotype combinations had higher body weight than those with other haplotype combinations (P < 0.05). The results suggest that these SNPs in the MC3R gene might be useful genetic markers for marker-assisted selection and cattle breeding.

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References

  • Butler AA, Kesterson RA, Khong K, Cullen MJ, Pelleymounter MA, Dekoning J, Baetscher M, Cone RD (2000) A unique metabolic syndrome causes obesity in the melanocortin-3-receptor-deficient mouse. Endocrinology 141:3518–3521

    CAS  PubMed  Google Scholar 

  • Chen AS, Marsh DJ, Trumbauer ME, Frazier EG, Guan XM, Yu H, Rosenblum CI, Vongs A, Feng Y, Cao L, Metzger JM, Strack AM, Camacho RE, Mellin TN, Nunes CN, Min W, Fisher J, Gopal-Truter S, MacIntyre DE, Chen HY, Van der Ploeg LH (2000) Inactivation of the mouse melanocortin-3 receptor results in increased fat mass and reduced lean body mass. Nat Genet 26:97–102

    Article  CAS  PubMed  Google Scholar 

  • Clark SA, Hickey JM, Daetwyler HD, van der Werf JH (2012) The importance of information on relatives for the prediction of genomic breeding values and the implications for the makeup of reference data sets in livestock breeding schemes. Genet Sel Evol 44:4

    Article  PubMed Central  PubMed  Google Scholar 

  • Crow LF, Kimura MA (1970) Introduction to population genetics theory. Harper & Row, New York

    Google Scholar 

  • Gantz I, Konda Y, Tashiro T, Shimoto Y, Miwa H, Munzert G, Watson SJ, Del Valle J, Yamada T (1993) Molecular cloning of a novel melanocortin receptor. J Biol Chem 268:8246–8250

    CAS  PubMed  Google Scholar 

  • Gilbert RP, Bailey DR, Shannon NH (1993) Linear body measurements of cattle before and after 20 years of selection for postweaning gain when fed two different diets. J Anim Sci 71:1712–1720

    CAS  PubMed  Google Scholar 

  • Goddard ME, Hayes BJ (2007) Genomic selection. J Anim Breed Genet 124:323–330

    Article  CAS  PubMed  Google Scholar 

  • Huang M, Gao X, Li JY, Ren HY, Chen JB, Xu SZ (2010) Polymorphisms in MC4R gene and correlations with economic traits in cattle. Mol Biol Rep 37(8):3941–3944

    Article  CAS  PubMed  Google Scholar 

  • Huang M, Xu SZ, Li JY, Gao X, Chen JB (2011) The tissue profile of MC3R and MC4R and the association of their expression in adipose with backfat thickness in Chinese Simmental cattle. Acta Veterinaria et Zootechnica Sinica 42(4):489–494 (in Chinese)

    CAS  Google Scholar 

  • Irani BG, Xiang Z, Yarandi HN, Holder JR, Moore MC, Bauzo RM, Proneth B, Shaw AM, Millard WJ, Chambers JB, Benoit SC, Clegg DJ, Haskell-Luevano C (2011) Implication of the melanocortin-3 receptor in the regulation of food intake. Eur J Pharmacol 66:80–87

    Article  Google Scholar 

  • Jiang SW, Jacobsson L, Kerje S, Andersson L, Xiong YZ (2002) Studies of relationship between the melanocortin-3 receptor gene and body weight in chicken for high and low weight lines intercross. Yi Chuan Xue Bao 29:322–325

    CAS  PubMed  Google Scholar 

  • Li SP, Du ZH, Ning FY, Sun HX, Bai XJ (2008) Correlation analysis between MC3R and MC4R gene polymorphism and growth traits in pigeon. Yi Chuan 30:1333–1340

    Article  CAS  PubMed  Google Scholar 

  • Liu H, Tian W, Zan L, Wang H, Cui H (2010) Mutations of MC4R gene and its association with economic traits in Qinchuan cattle. Mol Biol Rep 37(1):535–540

    Article  CAS  PubMed  Google Scholar 

  • Matukumalli LK, Lawley CT, Schnabel RD, Taylor JF, Allan MF, Heaton MP, O’Connell J, Moore SS, Smith TP, Sonstegard TS, Van Tassell CP (2009) Development and characterization of a high density SNP genotyping assay for cattle. PLoS ONE 4:e5350

    Article  PubMed Central  PubMed  Google Scholar 

  • Mencarelli M, Dubern B, Alili R, Maestrini S, Benajiba L, Tagliaferri M, Galan P, Rinaldi M, Simon C, Tounian P, Hercberg S, Liuzzi A, Di Blasio AM, Clement K (2011) Rare melanocortin-3 receptor mutations with in vitro functional consequences are associated with human obesity. Hum Mol Genet 20:392–399

    Article  CAS  PubMed  Google Scholar 

  • Müller A, Möller M, Adams LA, Warren RM, Hoal EG, van Helden PD (2012) Comparative analysis of a putative tuberculosis-susceptibility gene, MC3R, and pseudogene sequences in cattle, African buffalo, hyena, rhinoceros and other African bovids and ruminants. Cytogenet Genome Res 136:117–122

    Article  PubMed  Google Scholar 

  • Nei M, Roychoudhurg AK (1974) Sampling variance of heterozygosity and genetic distance. Genetics 76(2):379–390

    CAS  PubMed  Google Scholar 

  • Renquist BJ, Lippert RN, Sebag JA, Ellacott KL, Cone RD (2011) Physiological roles of the melanocortin MC3 receptor. Eur J Pharmacol 660:13–20

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Sambrook J, Russell DW (2002) In: Huang PT (ed) Molecular cloning: a laboratory manual, 3rd edn. Science Press, Beijing

    Google Scholar 

  • Santos JL, De la Cruz R, Holst C, Grau K, Naranjo C, Maiz A, Astrup A, Saris WH, MacDonald I, Oppert JM, Hansen T, Pedersen O, Sorensen TI, Martinez JA, Nugenob C (2011) Allelic variants of melanocortin 3 receptor gene (MC3R) and weight loss in obesity: a randomized trial of hypo-energetic high- versus low-fat diets. PLoS ONE 6:e19934

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • SAS Institute (1999) Statistical analysis systems user’s guide (Version 8). SAS Institute, Cary, NC

    Google Scholar 

  • Schwerin M (2003) Functional genomics: new possibilities for animal breeding and husbandry. Arch Tierz 46(Special issue):89–93

    Google Scholar 

  • Seong J, Suh DS, Park KD, Lee HK, Kong HS (2012) Identification and analysis of MC4R polymorphisms and their association with economic traits of Korean cattle (Hanwoo). Mol Biol Rep 39(4):3597–3601

    Article  CAS  PubMed  Google Scholar 

  • Sharma P, Bottje W, Okimoto R (2008) Polymorphisms in uncoupling protein, melanocortin 3 receptor, melanocortin 4 receptor, and pro-opiomelanocortin genes and association with production traits in a commercial broiler line. Poult Sci 87:2073–2786

    Article  CAS  PubMed  Google Scholar 

  • Sherman EL, Nkrumah JD, Moore SS (2010) Whole genome single nucleotide polymorphism associations with feed intake and feed efficiency in beef cattle. J Anim Sci 88:16–22

    Article  CAS  PubMed  Google Scholar 

  • Shi YY, He L (2005) SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res 15:97–98

    Article  CAS  PubMed  Google Scholar 

  • Skorczyk A, Flisikowski K, Szydlowski M, Cieslak J, Fries R, Switonski M (2011) Association of MC3R gene polymorphisms with body weight in the red fox and comparative gene organization in four canids. Anim Genet 42:104–107

    Article  CAS  PubMed  Google Scholar 

  • Wang XP, Luoreng ZM, Li F, Wang JR, Li N, Li SH (2012) Genetic polymorphisms of lipoprotein lipase gene and their associations with growth traits in Xiangxi cattle. Mol Biol Rep 39:10331–10338

    Article  CAS  PubMed  Google Scholar 

  • Zhang CL, Wang YH, Chen H, Lan XY, Lei CZ, Fang XT (2009) Association between variants in the 5′-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle. Mol Biol Rep 36(7):1839–1843

    Article  CAS  PubMed  Google Scholar 

  • Zhang CL, Wang YH, Lei CZ, Fang XT, Chen H (2011) The polymorphisms of bovine melanocortin-3 receptor pseudogene. Res Vet Sci 90:241–244

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We thank the reviewers and editor for helpful comments. This work was supported by the Open Scientific Research Project in the Provincial Platform of Life Sciences College, Hunan University of Arts and Science, China (SKYPT201207), the Natural Science Foundation of Hunan Province, China (11JJ4020), the Open Fund Project of Key Laboratory in Hunan Higher Education, China (11K045), the PhD Introduction Program of Hunan University of Arts and Science (BSQD1002), the Science-Technology Program Research Foundation of Hunan Province, China (2010NK3043), the Construct Program of the Key Discipline in Hunan Province.

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Authors and Affiliations

  1. Key Laboratory of Zoology in Hunan Higher Education, College of Life Science, Hunan University of Arts and Science, Changde, 415000, China

    Zhuo Ma Luoreng, Xing Ping Wang, Feng Li, Dong Sheng Guo, Na Li & Jin Ren Wang

  2. College of Life Sciences, Xinyang Normal University, Xinyang, 464000, Henan, China

    Yun Ma

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  1. Zhuo Ma Luoreng
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  2. Xing Ping Wang
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  4. Feng Li
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  7. Jin Ren Wang
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Correspondence to Xing Ping Wang.

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Zhuo Ma Luoreng and Xing Ping Wang have contributed equally to this work.

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Luoreng, Z.M., Wang, X.P., Ma, Y. et al. Three Novel SNPs in the Coding Region of the Bovine MC3R Gene and Their Associations with Growth Traits. Biochem Genet 52, 116–124 (2014). https://doi.org/10.1007/s10528-013-9632-7

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  • DOI: https://doi.org/10.1007/s10528-013-9632-7

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