Abstract
Carbonic anhydrase-related protein XI (CA-RP XI) is a member of the α-carbonic anhydrase family (encoded by the gene CA-11), which has lost features of the active site required for enzymatic activity. Using PCR, we amplified CA-11 from genomic DNA of the bat Megaderma lyra. To elucidate the gene structure, we sequenced PCR products and compared their sequences with genomic and mRNA sequences known from human and domestic pig. We identified and sequenced eight introns in the bat CA-11. Five introns (introns 3–7) are located in identical or similar positions in other members of the vertebrate α-carbonic anhydrase gene family. Two 5′ introns and one 3′ intron are located in the regions of little or no sequence similarity with other members of the gene family. The low sequence similarity and additional introns suggest a separate evolutionary origin for the 5′ and 3′ portions of the CA-RP XI gene.
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Acknowledgments
The tissue samples of M. lyra were provided by the Genetic Resources Collection of the Natural Science Research Laboratory of the Museum of Texas Tech University. We thank the field workers (listed in Hood et al. 1988) who collected the specimens used in the study. Electrophoresis of cycle sequencing reactions was performed by the DNA Sequence Core of the University of Michigan Medical School, by the Keck Laboratory at the University of New Orleans, and in the laboratory of Robert J. Baker at Texas Tech University. Karin Wiebauer provided helpful discussions and input. Robin Rowe of the University of New Orleans assisted with sequencing and provided valuable laboratory advice. The Biomedical Research Core Facilities at the University of Michigan Medical School synthesized primers used in the preliminary study. Funding was provided by a grant from the National Institutes of Health (GM-24681) to RET. The study also benefited from funding to CAP from the United Negro College Fund, the Research Initiative for Scientific Enhancement funded by the National Institutes of Health, and the Center for Undergraduate Research at Xavier University of Louisiana. The completion of this study was stimulated by the Morris Goodman Memorial Molecular Phylogenetics and Evolution Symposium held at Wayne State University in October 2011. The authors recognize Morris’s work as a colleague and collaborator on a variety of studies over many years.
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Porter, C.A., Hewett-Emmett, D. & Tashian, R.E. Carbonic Anhydrase-Related Protein XI: Structure of the Gene in the Greater False Vampire Bat (Megaderma lyra) Compared with Human and Domestic Pig. Biochem Genet 51, 474–481 (2013). https://doi.org/10.1007/s10528-013-9578-9
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DOI: https://doi.org/10.1007/s10528-013-9578-9