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MicroRNAs as Markers for Neurally Committed CD133+/CD34+ Stem Cells Derived from Human Umbilical Cord Blood

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Abstract

Neural differentiation of the CD133+/CD34+ subpopulation of human umbilical cord blood stem cells was investigated, and neuro-miR (mir-9 and mir-124) expression was examined. An efficient induction protocol for neural differentiation of hematopoietic stem cells together with the exclusion of retinoic acid in this process was also studied. Transcription of some neural markers such as microtubule-associated protein-2, beta-tubulin III, and neuron-specific enolase was evaluated by real-time PCR, immunocytochemistry, and western blotting. Increased expression of neural indicators in the treated cells confirmed the appropriate neural differentiation, which supported the high efficiency of our defined neuronal induction protocol. Verified high expression of neuro-miRNAs along with neuronal specific proteins not only strengthens the regulatory role of miRNAs in determining stem cell fate but also introduces these miRNAs as novel indicators of neural differentiation. These data highlight the prominent therapeutic potential of hematopoietic stem cells for use in cell therapy of neurodegenerative diseases.

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Acknowledgments

This article was financially supported by the Stem Cell Technology Research Center.

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Authors and Affiliations

  1. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Maryam Hafizi, Mahboubeh Kabiri & Samad Nadri

  2. Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran

    Amir Atashi & Masoud Soleimani

  3. Biology Department, Payame Noor University, Tehran, Iran

    Maryam Hafizi & Reza Haji Hosseini

  4. Department of Biotechnology, University College of Science, University of Tehran, Tehran, Iran

    Behnaz Bakhshandeh & Mahboubeh Kabiri

  5. Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Samad Nadri

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  1. Maryam Hafizi
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  2. Amir Atashi
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  3. Behnaz Bakhshandeh
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  7. Masoud Soleimani
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Correspondence to Masoud Soleimani.

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Hafizi, M., Atashi, A., Bakhshandeh, B. et al. MicroRNAs as Markers for Neurally Committed CD133+/CD34+ Stem Cells Derived from Human Umbilical Cord Blood. Biochem Genet 51, 175–188 (2013). https://doi.org/10.1007/s10528-012-9553-x

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  • DOI: https://doi.org/10.1007/s10528-012-9553-x

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