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MicroRNA signature associated with osteogenic lineage commitment

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Abstract

Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in several biological processes. miRNA microarray profiling was applied in order to identify the osteo-specific miRNA signature. Prior to this analysis, osteogenic commitment of osteoblasts was evaluated by measuring ALPase activity, biomineralization, specific staining and evaluation of some main osteogenic marker genes. To support our findings, various in silico explorations (for both putative targets and signaling pathways) and empirical analyses (miRNA transfections followed by qPCR of osteogenic indicators and ALPase activity measurement) were carried out. The function of GSK-3b inhibitor was also studied to investigate the role of WNT in osteogenesis. Transient modulation of multiple osteo-miRs (such as mir-199b, 1274a, 30b) with common targets (such as BMPR, TCFs, SMADs) as mediators of osteogenic pathways including cell–cell interactions, WNT and TGF-beta pathways, suggests a mechanism for rapid induction of the osteogenesis as an anti-miRNA therapy. The results of this research have identified the miRNA signature which regulates the osteogenesis mechanism in USSC. To conclude, our study reveals more details about the allocation of USSCs into osteogenic lineage through modulatory effect of miRNAs on targets and pathways required for creating a tissue-specific phenotype and may aid in future clinical interventions.

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Acknowledgments

This work was supported financially by Stem Cell Biology Department-Stem Cell Technology Research Center. We appreciate Mr. Seyed Zarvan Shahrzad for his contribution in English editing.

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Authors and Affiliations

  1. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

    Behnaz Bakhshandeh

  2. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

  3. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Behnaz Bakhshandeh & Maryam Hafizi

  4. School of Biology, Damghan University, Damghan, Iran

    Seyed Hassan Paylakhi

  5. School of Chemistry, College of Science, University of Tehran, Tehran, Iran

    Nasser Ghaemi

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  1. Behnaz Bakhshandeh
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  2. Masoud Soleimani
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  4. Seyed Hassan Paylakhi
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  5. Nasser Ghaemi
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Correspondence to Masoud Soleimani.

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Bakhshandeh, B., Soleimani, M., Hafizi, M. et al. MicroRNA signature associated with osteogenic lineage commitment. Mol Biol Rep 39, 7569–7581 (2012). https://doi.org/10.1007/s11033-012-1591-2

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