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Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant

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Abstract

Fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (Solanum melongena). ISSR and RAPD markers were used to characterize Fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern Turkey. Those isolates were not pathogenic to tomato. Pathogens were identified by their morphology, and their identity was confirmed by PCR amplification using the specific primer PF02-3. The isolates were classified into groups on the basis of ISSR and RAPD fingerprints, which showed a level of genetic specificity and diversity not previously identified in Fusarium oxysporum f. melongenae, suggesting that genetic differences are related to the pathogen in the Mediterranean region. The primers selected to characterize Fusarium oxysporum f. melongenae may be used to determine genetic differences and pathogen virulence. This study is the first to characterize eggplant F.oxysporum species using ISSR and RAPD.

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Acknowledgments

The authors wish to thank Dr. Francesco Mercati and Dr. Paolo Ruisi for their assistance with molecular studies carried out at the CNR Institute of Plant Genetics; Palermo, Italy; student F. Akay in the BATEM Molecular Biology Laboratory; technical assistant N. Karatekin for helping with studies carried out in climatic rooms; and Dr. F. Boyacı of BATEM for providing the Fusarium oxysporum f. melongenae reference isolate obtained from Italy.

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Authors and Affiliations

  1. Plant Pathology and Molecular Biology Department, West Mediterranean Agricultural Research Institute (BATEM), P.B. 35, 07100, Antalya, Turkey

    Ö. Baysal, E. Gümrükcü & S. Zengin

  2. Research Division Palermo, Institute of Plant Genetics, CNR, Corso Calatafimi 414, 90129, Palermo, Italy

    M. Siragusa & F. Carimi

  3. Botanic Science Department, University of Palermo, Via Archirafi 38, 90123, Palermo, Italy

    M. Sajeva

  4. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Jaime A. Teixeira da Silva

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  1. Ö. Baysal
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  2. M. Siragusa
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  3. E. Gümrükcü
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  4. S. Zengin
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  5. F. Carimi
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  6. M. Sajeva
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  7. Jaime A. Teixeira da Silva
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Correspondence to Ö. Baysal.

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Baysal, Ö., Siragusa, M., Gümrükcü, E. et al. Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant. Biochem Genet 48, 524–537 (2010). https://doi.org/10.1007/s10528-010-9336-1

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  • DOI: https://doi.org/10.1007/s10528-010-9336-1

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