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Genetic diversity and identification of race 3 of Fusarium oxysporum f. sp. lactucae in Taiwan

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Abstract

Fusarium wilt disease of lettuce (Lactuca sativa L.) is one of the major threats to the production of lettuce in Taiwan. This disease is caused by the vascular wilt pathogen Fusarium oxysporum f. sp. lactucae. It is important for the lettuce breeders to identify the pathological races of F. oxysporum f. sp. lactucae in the course of developing F. oxysporum f. sp. lactucae-resistant cultivars for growers. Recently, race 3 of F. oxysporum f. sp. lactucae was identified in Taiwan based on the results of pathogenicity tests against three differential lettuce cultivars, Patriot, Costa Rica No. 4, and Banchu Red Fire, along with some Japanese F. oxysporum f. sp. lactucae reference strains for comparison. In our collected F. oxysporum f. sp. lactucae isolates, most isolates from Taiwan were identified as race 1 except two isolates, Fola-10 and Fola-40 collected from Taoyuan County identified as race 3. This is the first report of F. oxysporum f. sp. lactucae race 3 outside of Japan. The PCR-based tagging technique with the four reference markers was used to confirm the results of pathogenicity tests. In addition, genetic diversity between races of F. oxysporum f. sp. lactucae was also observed according to the results of random amplification of polymorphic DNA (RAPD) fingerprintings.

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Acknowledgments

We are grateful to Drs. M. Fujinaga (Nagano Vegetable and Ornamental Crops Experiment Station, Japan), L. J. du Toit (Washington State University, USA), Y. S. Lin (Department of Plant Pathology, National Chung Hsing University, Taiwan, R.O.C.), K. S. Chen (Fengshan Tropical Horticultural Experiment Branch, Agricultural Research Institute, Taiwan, R.O.C.), C. H. Kuo (Department of Plant Medicine, National Chiayi University, Taiwan, R.O.C.), Mr. H. Y. Wu (Taoyuan District Agricultural Research and Extension Station, Taiwan, R.O.C.), National Institute of Agrobiological Sciences (Japan), AVRDC-The World Vegetable Center (Taiwan, R.O.C.), and Taitung District Agricultural Research and Extension Station (Taiwan, R.O.C.) for providing tested microorganisms. We thank Dr. C. J. Chang, Professor Emeritus, University of Georgia, for English editing of the manuscripts. This research was supported in part by Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture (Taiwan, R.O.C.) under grant numbers 95AS-13.3.1-BQ-B1(6), 96AS-14.3.1-BQ-B1(6), 97AS-14.3.1-BQ-B1(11), and 99AS-9.3.1-BQ-B2(6); by the Ministry of Science and Technology (formerly National Science Council), Taiwan, R.O.C. under grant numbers NSC-98-2313-B-005-025-MY3, NSC-99-2622-B-005-006-CC2, NSC-101-2313-B-005-028-MY3, NSC-101-2911-I-005-301, NSC-102-2911-I-005-301, and NSC-103-2911-I-005-301; by the Ministry of Education, Taiwan, R.O.C. under the ATU plan; and also by National Chung Hsing University, Taiwan, R.O.C. Dr. Ying-Hong Lin was supported with the postdoctoral fellowships provided by the Ministry of Science and Technology (formerly National Science Council), Taiwan, R.O.C. under grant numbers NSC-098-2811-B-005-024, NSC-099-2811-B-005-025, NSC-100-2811-B-005-017, NSC-101-2811-B-005-011, and NSC-102-2811-B-005-015.

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Authors and Affiliations

  1. Department of Plant Medicine, National Pingtung University of Science and Technology, Pingtung County, Taiwan, 91201, Republic of China

    Ying-Hong Lin

  2. Department of Plant Pathology, National Chung Hsing University, Taichung City, Taiwan, 40227, Republic of China

    Po-Jung Lai, Tao-Ho Chang, Yu-Ling Wan, Jenn-Wen Huang & Pi-Fang Linda Chang

  3. Agricultural Biotechnology Center, National Chung Hsing University, Taichung City, Taiwan, 40227, Republic of China

    Jenn-Wen Huang & Pi-Fang Linda Chang

  4. NCHU-UCD Plant and Food Biotechnology Center, National Chung Hsing University, Taichung City, Taiwan, 40227, Republic of China

    Jenn-Wen Huang & Pi-Fang Linda Chang

  5. Plant Pathology Division, Taiwan Agricultural Research Institute, Council of Agriculture, Wufeng District, Taichung City, Taiwan, 41362, Republic of China

    Jin-Hsing Huang

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  1. Ying-Hong Lin
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Lin, YH., Lai, PJ., Chang, TH. et al. Genetic diversity and identification of race 3 of Fusarium oxysporum f. sp. lactucae in Taiwan. Eur J Plant Pathol 140, 721–733 (2014). https://doi.org/10.1007/s10658-014-0493-7

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