Skip to main content
SpringerLink
Log in

Novel Mutations of the FASN Gene and Their Effect on Fatty Acid Composition in Japanese Black Beef

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

Eight novel and four known mutations were detected in the coding sequence of the bovine fatty acid synthase (FASN) gene of an F2 population from Japanese Black and Limousin cattle. Two mutations, g.16024A>G and g.16039T>C, detected in exon 34, which determine amino acid substitutions of threonine (T) to alanine (A) and tryptophan (W) to arginine (R), were clearly separated in the parental breeds. The haplotypes (TW and AR) segregated in F2 individuals and had a significant effect on the fatty acid composition of backfat, intermuscular fat, and intramuscular fat. The TW haplotype was associated with increasing C18:0 and C18:1 content and the ratio of monounsaturated to saturated fatty acids, and decreasing C14:0, C14:1, C16:0, and C16:1 content. The two mutations were screened in two commercial Japanese Black half-sibling populations and similarly determined the contribution to the fatty acid composition of intramuscular fat. The frequency of the TW haplotype was markedly higher in Japanese Black (0.67) than in Holstein (0.17), Angus (0.02), and Hereford (0.07). We conclude that these mutations may contribute to the characteristic fatty acid composition of Japanese Black beef.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abe T, Saburi J, Hasebe H, Nakagawa T, Kawamura T, Saito K, Nade T, Misumi S, Okumura T, Kuchida K, Hayashi T, Nakane S, Mitsuhasi T, Nirasawa K, Sugimoto Y, Kobayashi E (2008) Bovine QTL analysis for growth, carcass, and meat quality traits in an F2 population from a cross between Japanese Black and Limousin. J Anim Sci 86:2821–2832

    Google Scholar 

  • Baró L, Fonollá J, Peña JL, Martínez-Férez A, Lucena A, Jiménez J, Boza JJ, López-Huertas E (2003) n-3 Fatty acids plus oleic acid and vitamin supplemented milk consumption reduces total and LDL cholesterol, homocysteine and levels of endothelial adhesion molecules in healthy humans. Clin Nutr 22:175–182

    Article  PubMed  Google Scholar 

  • Cameron ND, Enser MB (1991) Fatty acid composition of lipid in longissimus dorsi muscle of Duroc and British landrace pigs and its relationship with eating quality. Meat Sci 29:295–307

    Article  CAS  Google Scholar 

  • Chakravarty B, Gu Z, Chirala SS, Wakil SJ, Quiocho FA (2004) Human fatty acid synthase: structure and substrate selectivity of the thioesterase domain. Proc Natl Acad Sci USA 101:15567–15572

    Article  CAS  PubMed  Google Scholar 

  • Dryden FD, Marchello JA (1970) Influence of total lipid and fatty acid composition upon the palatability of three bovine muscles. J Anim Sci 31:36–41

    CAS  Google Scholar 

  • Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  PubMed  Google Scholar 

  • Hoashi S, Ashida N, Ohsaki H, Utsugi T, Sasazaki S, Taniguchi M, Oyama K, Mukai F, Mannen H (2007) Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle. Mamm Genome 18:880–886

    Article  CAS  PubMed  Google Scholar 

  • Keys A, Grand F, Anderson JT (1974) Bias and misrepresentation revised: perspective on saturated fat. Am J Clin Nutr 27:188–212

    CAS  PubMed  Google Scholar 

  • Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 5:105–132

    Article  Google Scholar 

  • Laborde FL, Mandell IB, Tosh JJ, Wilton JW, Buchanan-Smith JG (2001) Breed effects on growth performance, carcass characteristics, fatty acid composition, and palatability attributes in finishing steers. J Anim Sci 79:355–365

    CAS  PubMed  Google Scholar 

  • Maier T, Jenni S, Ban N (2006) Architecture of mammalian fatty acid synthase at 4.5 A resolution. Science 311:1258–1262

    Article  CAS  PubMed  Google Scholar 

  • Matsubara K, Inoue Y, Tahara K, Nakagawa K (1998) Quality of and consumer preference for marketing beef. Bull Hyogo Pre Agric Inst (Animal Husbandry) 34:10–15 (Japanese)

    Google Scholar 

  • May SG, Sturdivant CA, Lunt DK, Miller RK, Smith SB (1993) Comparison of sensory characteristics and fatty acid composition between Wagyu crossbred and Angus steers. Meat Sci 35:289–298

    Article  CAS  Google Scholar 

  • Melton SL, Black JM, Davis GW, Backus WR (1982) Flavor and selected chemical components of ground beef from steers backgrounded on pasture and fed corn up to 140 days. J Food Sci 47:699–704

    Article  CAS  Google Scholar 

  • Morris CA, Cullen NG, Glass BC, Hyndman DL, Manley TR, Hickey SM, McEwan JC, Pitchford WS, Bottema CD, Lee MA (2007) Fatty acid synthase effects on bovine adipose fat and milk fat. Mamm Genome 18:64–74

    Article  CAS  PubMed  Google Scholar 

  • Roy R, Taourit S, Zaragoza P, Eggen A, Rodellar C (2005) Genomic structure and alternative transcript of bovine fatty acid synthase gene (FASN): comparative analysis of the FASN gene between monogastric and ruminant species. Cytogenet Genome Res 111:65–73

    Article  CAS  PubMed  Google Scholar 

  • Roy R, Ordovas L, Zaragoza P, Romero A, Moreno C, Altarriba J, Rodellar C (2006) Association of polymorphisms in the bovine FASN gene with milk-fat content. Anim Genet 37:215–218

    Article  CAS  PubMed  Google Scholar 

  • Smith S, Witkowski A, Joshi AK (2003) Structural and functional organization of the animal fatty acid synthase. Prog Lipid Res 42:289–317

    Article  CAS  PubMed  Google Scholar 

  • Taniguchi M, Utsugi T, Oyama K, Mannen H, Kobayashi M, Tanabe Y, Ogino A, Tsuji S (2004) Genotype of stearoyl-coA desaturase is associated with fatty acid composition in Japanese Black cattle. Mamm Genome 15:142–148

    Article  CAS  PubMed  Google Scholar 

  • Temme EH, Mensink RP, Hornstra G (1997) Effects of medium chain fatty acids (MCFA), myristic acid, and oleic acid on serum lipoproteins in healthy subjects. J Lipid Res 38:1746–1754

    CAS  PubMed  Google Scholar 

  • Westerling DB, Hedrick HB (1979) Fatty acid composition of bovine lipids as influenced by diet, sex and anatomical location and relationship to sensory characteristics. J Anim Sci 48:1343–1348

    CAS  Google Scholar 

  • Yoshimura T, Namikawa K (1985a) Influence of breed, sex and anatomical location on lipid and fatty acid composition of bovine intermuscular fat. Jpn J Zootech Sci 56:122–129

    CAS  Google Scholar 

  • Yoshimura T, Namikawa K (1985b) Influence of breed, sex and anatomical location on lipid and fatty acid composition of bovine body-cavity fat. Jpn J Zootech Sci 56:130–136

    CAS  Google Scholar 

  • Yoshimura T, Namikawa K (1985c) Influence of breed, sex and anatomical location on lipid and fatty acid composition of bovine visceral fat. Jpn J Zootech Sci 56:137–143

    CAS  Google Scholar 

  • Zembayashi M, Nishimura K, Lunt DK, Smith SB (1995) Effect of breed type and sex on the fatty acid composition of subcutaneous and intramusucular lipids of finishing steers and heifers. J Anim Sci 73:3325–3332

    CAS  PubMed  Google Scholar 

  • Zhang S, Knight TJ, Reecy JM, Beitz DC (2008) DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition. Anim Genet 39:62–70

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank the Shirakawa Institute of Animal Genetics for utilization of their automatic DNA extraction equipment, and the staff of the Tokachi and Ohu branches of the NLBC for meat sample collection from F2 animals.

Author information

Authors and Affiliations

  1. National Livestock Breeding Center, 1 Odakurahara, Nishigo, Fukushima, 961-8511, Japan

    Tsuyoshi Abe, Junich Saburi, Hiroyuki Hasebe, Tetsuo Nakagawa, Satsuki Misumi & Eiji Kobayashi

  2. Nippon Veterinary and Life Science University, Musashino, Tokyo, 180-8602, Japan

    Toshihiro Nade

  3. Yamagata Prefectural Animal Industrial Institute, Agricultural Research Center, 1076 Torigoe, Shinjo, Yamagata, 996-0041, Japan

    Hiroaki Nakajima, Noriaki Shoji & Masato Kobayashi

Authors
  1. Tsuyoshi Abe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Junich Saburi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroyuki Hasebe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tetsuo Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Satsuki Misumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toshihiro Nade
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hiroaki Nakajima
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Noriaki Shoji
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Masato Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Eiji Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tsuyoshi Abe.

Additional information

Tsuyoshi Abe and Junich Saburi have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abe, T., Saburi, J., Hasebe, H. et al. Novel Mutations of the FASN Gene and Their Effect on Fatty Acid Composition in Japanese Black Beef. Biochem Genet 47, 397–411 (2009). https://doi.org/10.1007/s10528-009-9235-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-009-9235-5

Keywords

Search

Navigation